Wüst J, Hardegger U
Department of Clinical Microbiology, University of Zurich, Switzerland.
Eur J Clin Microbiol Infect Dis. 1992 Dec;11(12):1169-73. doi: 10.1007/BF01961139.
The susceptibility of 146 recent clinical isolates of gram-negative and gram-positive anaerobes was determined by the E test (AB Biodisk) on both Wilkins-Chalgren and PDM ASM II (AB Biodisk) agar. Results of the E test were compared with results obtained by the NCCLS agar dilution method using Wilkins-Chalgren agar. Incubation was for 20 hours and 44 hours in the E test and for 44 hours in the NCCLS method. In general, 44 hour results were more reliable; however, NCCLS readings were made only once after 44 hours. After two days of incubation, 91% of E test results on Wilkins-Chalgren agar were within one dilution and 98% within two dilutions of the corresponding NCCLS values; on PDM agar these values were 89% and 98%, respectively. Major and very major discrepancies combined were less than 1%.
采用E试验（AB生物盘）在威尔金斯-查尔格伦琼脂和PDM ASM II（AB生物盘）琼脂上测定了146株近期临床分离的革兰氏阴性和革兰氏阳性厌氧菌的药敏性。将E试验结果与使用威尔金斯-查尔格伦琼脂的NCCLS琼脂稀释法获得的结果进行比较。E试验的孵育时间为20小时和44小时，NCCLS方法的孵育时间为44小时。一般来说，44小时的结果更可靠；然而，NCCLS读数仅在44小时后进行一次。孵育两天后，威尔金斯-查尔格伦琼脂上91%的E试验结果在相应NCCLS值的一个稀释度范围内，98%在两个稀释度范围内；在PDM琼脂上，这些值分别为89%和98%。主要和非常主要的差异合并起来不到1%。
