Bauréus Koch C L M, Sommarin M, Persson B R R, Salford L G, Eberhardt J L
Department of Radiation Physics, Lund University Hospital, Lund, Sweden.
Bioelectromagnetics. 2003 Sep;24(6):395-402. doi: 10.1002/bem.10136.
The question of whether very weak low frequency magnetic fields can affect biological systems, has attracted attention by many research groups for quite some time. Still, today, the theoretical possibility of such an interaction is often questioned and the site of interaction in the cell is unknown. In the present study, the influence of extremely low frequency (ELF) magnetic fields on the transport of Ca(2+) was studied in a biological system consisting of highly purified plasma membrane vesicles. We tested two quantum mechanical theoretical models that assume that biologically active ions can be bound to a channel protein and influence the opening state of the channel. Vesicles were exposed for 30 min at 32 degrees C and the calcium efflux was studied using radioactive (45)Ca as a tracer. Static magnetic fields ranging from 27 to 37 micro T and time varying magnetic fields with frequencies between 7 and 72 Hz and amplitudes between 13 and 114 micro T (peak) were used. We show that suitable combinations of static and time varying magnetic fields directly interact with the Ca(2+) channel protein in the cell membrane, and we could quantitatively confirm the model proposed by Blanchard.
极低频弱磁场是否会影响生物系统这一问题，已经吸引众多研究团队关注了相当长一段时间。即便如此，时至今日，这种相互作用的理论可能性仍常遭质疑，且细胞内的相互作用位点也尚不明确。在本研究中，我们在由高度纯化的质膜囊泡构成的生物系统中，研究了极低频（ELF）磁场对Ca(2+)转运的影响。我们测试了两种量子力学理论模型，这两种模型假定具有生物活性的离子可与通道蛋白结合并影响通道的开放状态。囊泡在32摄氏度下暴露30分钟，并用放射性（45）Ca作为示踪剂研究钙外流情况。使用了强度范围在27至37微特斯拉的静磁场以及频率在7至72赫兹、幅度在13至114微特斯拉（峰值）之间的时变磁场。我们发现，静磁场和时变磁场的适当组合可直接与细胞膜中的Ca(2+)通道蛋白相互作用，并且我们能够定量证实布兰查德提出的模型。
