Boyle M J, Sculley T B, Cooper D A, Turner J J, Penny R, Sewell W A
Centre for Immunology, St Vincent's Hospital, Sydney, Australia.
Clin Exp Immunol. 1992 Mar;87(3):357-61. doi: 10.1111/j.1365-2249.1992.tb03002.x.
Persistent generalized lymphadenopathy (PGL) and polyclonal B cell activation are features of infection with HIV. Epstein-Barr virus (EBV) and HIV are known to activate B cells in vitro, but whether they are important B cell activators in patients infected with HIV is less clear. In this study, lymph node tissue was obtained from 10 patients with PGL and assessed for evidence of EBV and HIV gene sequences. DNA was extracted and specific viral gene sequences identified using the polymerase chain reaction (PCR). EBV sequences were difficult to detect in the PGL tissue, with a signal intensity similar to that of other benign and malignant lymphoid conditions not associated with EBV. HIV sequences were also rare in the PGL tissue, consistent with HIV infection of the small number of peripheral blood cells and nodal T cells likely to be present in such a sample. These findings suggest that the polyclonal B cell activation typical of HIV is not driven by direct EBV or HIV infection of B cells.
持续性全身性淋巴结肿大（PGL）和多克隆B细胞激活是HIV感染的特征。已知爱泼斯坦-巴尔病毒（EBV）和HIV可在体外激活B细胞，但它们是否为HIV感染患者重要的B细胞激活剂尚不清楚。在本研究中，从10例PGL患者获取淋巴结组织，并评估EBV和HIV基因序列的证据。提取DNA并使用聚合酶链反应（PCR）鉴定特定病毒基因序列。在PGL组织中难以检测到EBV序列，其信号强度与其他与EBV无关的良性和恶性淋巴样疾病相似。PGL组织中HIV序列也很罕见，这与该样本中可能存在的少量外周血细胞和淋巴结T细胞的HIV感染情况一致。这些发现表明，HIV典型的多克隆B细胞激活并非由B细胞直接感染EBV或HIV所致。
