用于快速检测人巨细胞病毒感染的微量滴定板荧光抗体检测法的评估
Evaluation of a microtiter plate fluorescent-antibody assay for rapid detection of human cytomegalovirus infection.
作者信息
Boppana S B, Smith R J, Stagno S, Britt W J
机构信息
Department of Pediatrics and Microbiology, University of Alabama, Birmingham 35294-0011.
出版信息
J Clin Microbiol. 1992 Mar;30(3):721-3. doi: 10.1128/jcm.30.3.721-723.1992.
Abstract
The use of monoclonal antibody (MAb) p63-27, which is reactive with the major immediate-early human cytomegalovirus (HCMV) protein pp72, was explored for the rapid diagnosis of HCMV viruria. The rapid assay detected all but 1 of 19 specimens identified by standard virus isolation methods from 1,676 newborn urine specimens, achieving a sensitivity of 94.5% and a specificity of 100%. The monoclonal antibody recognized 260 randomly obtained clinical isolates of HCMV, indicating the suitability of this reagent for use in screening assays. The sensitivity of the microtiter plate method declined rapidly for specimen from older infants and children with congenital CMV infection and virus-infected children attending a day-care center and was judged to be unacceptable for screening populations in this age group.
摘要
研究了与人类巨细胞病毒（HCMV）主要立即早期蛋白pp72反应的单克隆抗体（MAb）p63-27用于快速诊断HCMV病毒尿的情况。该快速检测法在1676份新生儿尿液标本中，检测出了标准病毒分离方法鉴定的19份标本中的18份，灵敏度达到94.5%，特异性为100%。该单克隆抗体识别了260株随机获得的HCMV临床分离株，表明该试剂适用于筛查检测。对于先天性CMV感染的大龄婴儿和儿童以及日托中心的病毒感染儿童的标本，微量滴定板法的灵敏度迅速下降，被认为不适用于该年龄组人群的筛查。
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