Mueller A J, Klauss V, Gürtler L, Geier S
Augenklinik der Universität, München, Germany.
Infection. 1992 Sep-Oct;20(5):249-52. doi: 10.1007/BF01710788.
Beside the risk of infection via HIV-1-contaminated blood, ophthalmologists are especially interested in the possibility of HIV-1 infection via tears. Therefore we tried to isolate HIV-1 from tears of 50 HIV-1-infected persons in different stages of disease by reverse transcriptase (RT) and by p24-antigen (p24-AG) in the cultures. Simultaneously we tried to isolate HIV-1 in the supernatant from peripheral blood lymphocytes (PBL), which was successful in 32 of the 50 examined specimens. HIV-1 could not be isolated from the tears of these persons. In addition, polymerasechain-reaction (PCR) was performed to detect proviral sequences (gag, pol, env) of HIV-1 in tears and blood of ten HIV-1-infected patients. While in all the examined patients gag, pol and env could be detected in the blood samples, only one tear sample was found positive for gag and pol DNA fragments. These results indicate that tears of HIV-1-positive contain extremely low quantities of tissue culture infectious doses (TCID) of HIV-1 in contrast to PBL. HIV-1 infection via tears therefore appears to be unlikely.
除了通过受HIV-1污染的血液感染的风险外，眼科医生尤其关注通过眼泪感染HIV-1的可能性。因此，我们试图通过逆转录酶（RT）和培养物中的p24抗原（p24-AG），从50名处于不同疾病阶段的HIV-1感染者的眼泪中分离出HIV-1。同时，我们试图从外周血淋巴细胞（PBL）的上清液中分离出HIV-1，在所检测的50个标本中有32个成功分离出。从这些人的眼泪中未能分离出HIV-1。此外，对10名HIV-1感染患者的眼泪和血液进行了聚合酶链反应（PCR），以检测HIV-1的前病毒序列（gag、pol、env）。虽然在所有检测患者的血液样本中都能检测到gag、pol和env，但仅在一个眼泪样本中发现gag和pol DNA片段呈阳性。这些结果表明，与PBL相比，HIV-1阳性者的眼泪中含有极低数量的HIV-1组织培养感染剂量（TCID）。因此，通过眼泪感染HIV-1似乎不太可能。
