Kunieda T, Kobayashi E, Tachibana M, Ikadai H, Imamichi T
Imamichi Institute for Animal Reproduction, Ibaraki, Japan.
Mamm Genome. 1992;3(10):564-7. doi: 10.1007/BF00350622.
The EMBL and GenBank DNA databases were searched for microsatellite sequences of the rat containing dinucleotide repeats of (CA)n and (GA)n. Among those obtained, 23 sequences were analyzed by polymerase chain reaction to examine the size variation of the amplified fragment in inbred rat strains. All of the 23 microsatellite sequences varied in size among the strains tested. The 23 microsatellite loci in a pair of substrains separated from the same progenitor strain were then analyzed. Fragments identical in size were observed in all loci of the two substrains, indicating the stability of the microsatellite over a large number of generations. The microsatellite loci, therefore, should be useful markers for linkage analyses in the rat.
在欧洲分子生物学实验室（EMBL）和基因银行（GenBank）的DNA数据库中搜索含有（CA）n和（GA）n二核苷酸重复序列的大鼠微卫星序列。在获得的序列中，通过聚合酶链反应分析了23个序列，以检测近交系大鼠品系中扩增片段的大小变异。在所测试的品系中，所有23个微卫星序列的大小均有所不同。然后分析了从同一祖系品系分离出的一对亚系中的23个微卫星位点。在两个亚系的所有位点中均观察到大小相同的片段，这表明微卫星在大量世代中具有稳定性。因此，微卫星位点应是大鼠连锁分析的有用标记。
