Stevens T R, Winrow V R, Blake D R, Rampton D S
Gastrointestinal Unit, London Hospital Medical College, UK.
Clin Exp Immunol. 1992 Nov;90(2):271-4. doi: 10.1111/j.1365-2249.1992.tb07941.x.
Heat-shock proteins (HSPs) are highly conserved immunogenic intracellular molecules that are induced by inflammatory mediators and may induce autoimmune phenomena in vivo. We have recently demonstrated the increased expression of HSP-60 in the colonocytes of patients with ulcerative colitis. To study further the role of HSP-60 in inflammatory bowel disease, we have now measured antibodies to recombinant mycobacterial HSP-65 (a member of the HSP-60 family) in patients with Crohn's disease, ulcerative colitis, healthy volunteers and, as disease controls, patients with confirmed bacterial diarrhoea. In comparison with healthy controls (n = 20; median level of 89 ELISA units; range 24-292), serum IgA HSP-60 antibodies were elevated in Crohn's disease (n = 21; 157; 57-364; P < 0.05) and active ulcerative colitis (n = 16; 188; 58-373; P < 0.01) but not bacterial diarrhoea (n = 10; 106; 51-285). Increased IgA HSP-60 antibody levels in patients with inflammatory bowel disease may occur as the result of HSP release from injured gut epithelium; alternatively, increased intestinal permeability could facilitate mucosal access of luminal antigens and the generation of cross-reactive anti-bacterial HSP antibodies.
热休克蛋白（HSPs）是高度保守的免疫原性细胞内分子，由炎症介质诱导产生，可能在体内诱发自身免疫现象。我们最近已证实溃疡性结肠炎患者结肠细胞中HSP - 60的表达增加。为了进一步研究HSP - 60在炎症性肠病中的作用，我们现在检测了克罗恩病、溃疡性结肠炎患者、健康志愿者以及确诊为细菌性腹泻的患者（作为疾病对照）体内针对重组分枝杆菌HSP - 65（HSP - 60家族成员）的抗体。与健康对照者（n = 20；酶联免疫吸附测定（ELISA）单位中位数水平为89；范围24 - 292）相比，克罗恩病患者（n = 21；157；57 - 364；P < 0.05）和活动期溃疡性结肠炎患者（n = 16；188；58 - 373；P < 0.01）血清IgA HSP - 60抗体升高，而细菌性腹泻患者（n = 10；106；51 - 285）则未升高。炎症性肠病患者体内IgA HSP - 60抗体水平升高可能是由于受损肠道上皮释放HSP所致；或者，肠道通透性增加可能会促进腔内抗原进入黏膜并产生交叉反应性抗细菌HSP抗体。
