Nwokolo C U, Bickley J, Attard A R, Owen R J, Costas M, Fraser I A
Department of Gastroenterology, Walsgrave Hospital, Coventry.
Gut. 1992 Oct;33(10):1323-7. doi: 10.1136/gut.33.10.1323.
Nine members of a family with a high incidence of duodenal ulcer disease were studied by interview, examination of hospital records, endoscopy, and antral biopsy. Helicobacter pylori was confirmed by CLO test, histology and culture. DNA extraction from pure isolates of H pylori was possible in six family members and strain typing was performed by restriction fragment length polymorphism. DNA restriction digestion was followed by vacublotting and then DNA hybridisation, using a cDNA probe complimentary to H pylori rRNA cistrons. Eight of the nine family members were H pylori positive by CLO test and histology. Five had duodenal ulcer disease. Three family members (one from each generation) harboured clonal variants of a single parent strain of H pylori but only two had duodenal disease. The other three members harboured different strains. Intrafamilial clustering of clonal variants of H pylori occurs in some duodenal ulcer disease families. Family members however, may develop duodenal disease irrespective of the colonising strain.
对一个十二指肠溃疡病高发家族的九名成员进行了研究，研究方法包括访谈、查阅医院记录、内镜检查和胃窦活检。通过CLO试验、组织学检查和培养确诊幽门螺杆菌。六名家族成员的幽门螺杆菌纯培养物可用于DNA提取，并通过限制性片段长度多态性进行菌株分型。DNA限制性消化后进行真空印迹，然后使用与幽门螺杆菌rRNA顺反子互补的cDNA探针进行DNA杂交。九名家族成员中有八名通过CLO试验和组织学检查显示幽门螺杆菌呈阳性。五名患有十二指肠溃疡病。三名家族成员（每代一名）携带单一亲本幽门螺杆菌菌株的克隆变体，但只有两名患有十二指肠疾病。其他三名成员携带不同的菌株。幽门螺杆菌克隆变体在家族内的聚集现象在一些十二指肠溃疡病家族中存在。然而，家族成员无论感染何种定植菌株都可能患十二指肠疾病。
