Matsuyama Hidetoshi, Hirabayashi Toshikazu, Kasahara Hirokazu, Minami Hideki, Hoshino Tamotsu, Yumoto Isao
Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
Department of Marine Science and Technology, School of Engineering, Hokkaido Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
Int J Syst Evol Microbiol. 2006 Dec;56(Pt 12):2883-2886. doi: 10.1099/ijs.0.64413-0.
Two novel exopolysaccharide-producing bacteria, strains S18K6(T) and S18K5, were isolated from Pacific Ocean sediment. The isolates were Gram-negative, motile, strictly aerobic chemoheterotrophic bacteria. The DNA G+C contents of strains S18K6(T) and S18K5 were 44.8 and 46.3 mol%, respectively. DNA-DNA relatedness between the two strains was 70 %. Major fatty acids were hexadecanoic acid (C(16 : 0)), hexadecenoic acid (C(16 : 1)omega7c) and octadecenoic acid (C(18 : 1)omega7c). 16S rRNA gene sequence, chemotaxonomic and morphological data indicated that these strains clearly belonged to the genus Glaciecola. Based on phenotypic properties and DNA-DNA hybridization data, strains S18K6(T) and S18K5 are considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola chathamensis sp. nov. is proposed. The type strain is S18K6(T) (=JCM 13645(T)=NCIMB 14146(T)).
从太平洋沉积物中分离出两株新型产胞外多糖细菌，菌株S18K6(T)和S18K5。分离菌株为革兰氏阴性、运动性、严格需氧的化能异养细菌。菌株S18K6(T)和S18K5的DNA G+C含量分别为44.8和46.3 mol%。两株菌之间的DNA-DNA相关性为70%。主要脂肪酸为十六烷酸（C(16 : 0)）、十六碳烯酸（C(16 : 1)ω7c）和十八碳烯酸（C(18 : 1)ω7c）。16S rRNA基因序列、化学分类和形态学数据表明，这些菌株明显属于嗜冷杆菌属。基于表型特征和DNA-DNA杂交数据，菌株S18K6(T)和S18K5被认为代表嗜冷杆菌属的一个新物种，为此提出了新名称嗜冷查塔姆杆菌（Glaciecola chathamensis sp. nov.）。模式菌株为S18K6(T)（=JCM 13645(T)=NCIMB 14146(T)）。
