Yong Jeong-Joong, Park Soo-Je, Kim Hyeon-Ju, Rhee Sung-Keun
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.
Deep Ocean Water Application Research Center, Korea Ocean Research and Development Institute (KORDI), 245-7 Oho-ri, Jugwang-myeon, Goseong-gun 219-822, Republic of Korea.
Int J Syst Evol Microbiol. 2007 May;57(Pt 5):951-953. doi: 10.1099/ijs.0.64723-0.
A taxonomic study was carried out on an isolate, strain NO2(T), from marine sediment collected from the East Sea, Korea. Comparative 16S rRNA gene sequence studies showed that this strain belonged to the Gammaproteobacteria and was most closely related to Glaciecola mesophila KMM 241(T) and Glaciecola polaris LMG 21857(T) (98.6 and 98.0 % 16S rRNA gene sequence similarity, respectively). The isolate was Gram-negative, aerobic and slightly halophilic and grew in 2-8 % NaCl and at 7-30 degrees C. Strain NO2(T) shared some physiological and biochemical properties with G. mesophila KMM 241(T) and G. polaris LMG 21857(T). The G+C content of the genomic DNA of strain NO2(T) was 45 mol%. Strain NO2(T) possessed C(16 : 0), summed feature 4 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH) and summed feature 7 (C(18 : 1)omega9c/omega12t/omega7c) as the major cellular fatty acids. DNA-DNA relatedness data indicated that strain NO2(T) represents a distinct species that is separate from G. mesophila and G. polaris. On the basis of polyphasic evidence, it is proposed that strain NO2(T) (=KCTC 12755(T)=LMG 23762(T)) represents the type strain of a novel species, Glaciecola agarilytica sp. nov.
对从韩国东海采集的海洋沉积物中分离得到的菌株NO2(T)进行了分类学研究。比较16S rRNA基因序列研究表明，该菌株属于γ-变形菌纲，与嗜中温嗜冷杆菌KMM 241(T)和极地嗜冷杆菌LMG 21857(T)关系最为密切（16S rRNA基因序列相似性分别为98.6%和98.0%）。该分离株为革兰氏阴性、需氧且轻度嗜盐，能在2%-8%的NaCl中以及7-30℃下生长。菌株NO2(T)与嗜中温嗜冷杆菌KMM 241(T)和极地嗜冷杆菌LMG 21857(T)具有一些生理生化特性。菌株NO2(T)基因组DNA的G+C含量为45 mol%。菌株NO2(T)的主要细胞脂肪酸为C(16 : 0)、总和特征4（C(16 : 1)ω7c和/或异-C(15 : 0) 2-OH）和总和特征7（C(18 : 1)ω9c/ω12t/ω7c）。DNA-DNA相关性数据表明，菌株NO2(T)代表一个与嗜中温嗜冷杆菌和极地嗜冷杆菌不同的独特物种。基于多相证据，提议将菌株NO2(T)（=KCTC 12755(T)=LMG 23762(T)）作为一个新物种——琼脂嗜冷杆菌（Glaciecola agarilytica sp. nov.）的模式菌株。
