Goldberg R J, Levin R, Parks W P, Scolnick E M
J Virol. 1975 Jan;17(1):43-50. doi: 10.1128/JVI.17.1.43-50.1976.
The specificity and quantitation of the rescue of RNA sequences by mammalian type C viruses has been investigated. Type C virus can package with specificity only type C viral RNA. Type C viruses do not encapsidate with comparable efficiency either type B viral or cellular globin mRNA. Conversely, a non-type C mammalian retravirus, MP-MV, cannot encapsidate type C RNA. A revertant of Kirsten sarcoma virus (Ki-SV)-transformed nonproducer cells which fails to rescue biologically active Ki-SV after superinfection with helper virus had no detectable intracellular Ki-SV-specific RNA. The results suggest specific mechanisms by which type C viral proteins can package type C viral RNA and provide an approach to classifying RNA of potentially defective endogenous retraviruses as type C in origin.
对哺乳动物C型病毒拯救RNA序列的特异性和定量进行了研究。C型病毒只能特异性地包装C型病毒RNA。C型病毒不能以相当的效率包装B型病毒RNA或细胞珠蛋白mRNA。相反,一种非C型哺乳动物逆转录病毒MP-MV不能包装C型RNA。经辅助病毒超感染后无法拯救具有生物活性的柯斯顿肉瘤病毒(Ki-SV)的Ki-SV转化的非生产细胞的回复体,没有可检测到的细胞内Ki-SV特异性RNA。这些结果提示了C型病毒蛋白包装C型病毒RNA的特定机制,并提供了一种将潜在缺陷的内源性逆转录病毒的RNA归类为C型起源的方法。