Sullivan R M, Wilson D A
University of Oklahoma, Norman, Oklahoma.
Psychobiology (Austin, Tex). 1991;19(4):308-312. doi: 10.3758/bf03332084.
In neonatal rats, norepinephrine (NE) is necessary and sufficient for the acquisition of an olfactory preference and its associated olfactory bulb neural modifications as assessed by [(14)C] 2-deoxyglucose (2-DG) and electrophysiology. In the present studies, we assessed the influence of NE on the expression of a conditioned odor preference and its associated olfactory bulb neural modifications in neonatal rats. On Postnatal Day 5 (PN 5), pups were trained to prefer an odor in a 1-h classical conditioning paradigm. Experimental paired odor-stroke pups received 20 forward pairings of a 10-sec peppermint odor and a 9-sec reinforcing tactile stimulation (stroking). Control pups received either random stroke-odor pairings or were naive (received neither the odor nor stroking). The next day (PN 6), the pups were injected with either an NE β-receptor antagonist, (propranolol or timolol) or saline, 1 h prior to testing. The pups were tested for a behavioral olfactory preference and assessed for differential olfactory bulb activity with [(14)C] 2-DG autoradiography. The results indicate that NE is not necessary for the expression of the learned neurobehavioral response.
在新生大鼠中，去甲肾上腺素（NE）对于获得嗅觉偏好及其相关的嗅球神经改变是必要且充分的，这通过[（14）C] 2-脱氧葡萄糖（2-DG）和电生理学评估得出。在本研究中，我们评估了NE对新生大鼠条件性气味偏好表达及其相关嗅球神经改变的影响。在出生后第5天（PN 5），幼崽在1小时的经典条件反射范式中被训练偏好一种气味。实验性配对气味-抚摸幼崽接受了20次10秒薄荷气味与9秒强化触觉刺激（抚摸）的正向配对。对照幼崽接受随机的抚摸-气味配对或未接受任何处理（既未接触气味也未接受抚摸）。第二天（PN 6），在测试前1小时，给幼崽注射NEβ受体拮抗剂（普萘洛尔或噻吗洛尔）或生理盐水。对幼崽进行行为嗅觉偏好测试，并通过[（14）C] 2-DG放射自显影评估嗅球的差异活动。结果表明，NE对于习得的神经行为反应的表达并非必要。
