Iranshahi Mehrdad, Kalategi Farhad, Rezaee Ramin, Shahverdi Ahmad Reza, Ito Chihiro, Furukawa Hiroshi, Tokuda Harukuni, Itoigawa Masataka
Department of Pharmacognosy and Biotechnology, Biotechnology Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Planta Med. 2008 Feb;74(2):147-50. doi: 10.1055/s-2008-1034293. Epub 2008 Jan 31.
Several natural products have been found to have anti-tumor promoting activity. In the present study, we carried out a primary screening of ten terpenoid coumarins isolated from plants of the Ferula species, examining their possible inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12- O-tetradecanoylphorbol 13-acetate (TPA) in Raji cells. Auraptene (7-geranyloxycoumarin, 1) and umbelliprenin (7-farnesyloxycoumarin, 2) were found to significantly inhibit EBV-EA activation and preserved the high viability of Raji cells, suggesting that they might be valuable anti-tumor-promoting agents (IC (50) 8.3 and 9.1 nM, respectively). Our findings revealed that the presence of a prenyl moiety in the terpenoid coumarins plays an important role in anti-tumor promoting activity as previously reported for xanthones, coumarins, flavonoids and phenylpropanoids.
已发现几种天然产物具有抗肿瘤促进活性。在本研究中，我们对从阿魏属植物中分离出的十种萜类香豆素进行了初步筛选，检测它们对12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的Raji细胞中爱泼斯坦 - 巴尔病毒早期抗原（EBV - EA）激活的可能抑制作用。发现奥瑞烯（7 - 香叶氧基香豆素，1）和伞形异戊烯基香豆素（7 - 法呢基氧基香豆素，2）能显著抑制EBV - EA激活并保持Raji细胞的高活力，表明它们可能是有价值的抗肿瘤促进剂（IC（50）分别为8.3和9.1 nM）。我们的研究结果表明，萜类香豆素中异戊烯基部分的存在在抗肿瘤促进活性中起重要作用，这与之前关于呫吨酮、香豆素、黄酮类化合物和苯丙素类化合物的报道一致。
