Dam J S, Pedersen C B, Dalgaard T, Fabricius P E, Aruna P, Andersson-Engels S
Appl Opt. 2001 Mar 1;40(7):1155-64. doi: 10.1364/ao.40.001155.
We present a compact, fast, and versatile fiber-optic probe system for real-time determination of tissue optical properties from spatially resolved continuous-wave diffuse reflectance measurements. The system collects one set of reflectance data from six source-detector distances at four arbitrary wavelengths with a maximum overall sampling rate of 100 Hz. Multivariate calibration techniques based on two-dimensional polynomial fitting are employed to extract and display the absorption and reduced scattering coefficients in real-time mode. The four wavelengths of the current configuration are 660, 785, 805, and 974 nm, respectively. Cross-validation tests on a 6 x 7 calibration matrix of Intralipid-dye phantoms showed that the mean prediction error at, e.g., 785 nm was 2.8% for the absorption coefficient and 1.3% for the reduced scattering coefficient. The errors are relative to the range of the optical properties of the phantoms at 785 nm, which were 0-0.3/cm for the absorption coefficient and 6-16/cm for the reduced scattering coefficient. Finally, we also present and discuss results from preliminary skin tissue measurements.
我们展示了一种紧凑、快速且通用的光纤探头系统，用于通过空间分辨连续波漫反射测量实时测定组织光学特性。该系统以100 Hz的最大总采样率，在四个任意波长下从六个源 - 探测器距离收集一组反射率数据。采用基于二维多项式拟合的多元校准技术，以实时模式提取并显示吸收系数和约化散射系数。当前配置的四个波长分别为660、785、805和974 nm。对脂质体 - 染料模型的6×7校准矩阵进行的交叉验证测试表明，例如在785 nm处，吸收系数的平均预测误差为2.8%，约化散射系数的平均预测误差为1.3%。这些误差相对于模型在785 nm处光学特性的范围，吸收系数的范围为0 - 0.3/cm，约化散射系数的范围为6 - 16/cm。最后，我们还展示并讨论了初步皮肤组织测量的结果。
