Kwakman Paulus H S, Van den Akker Johannes P C, Güçlü Ahmet, Aslami Hamid, Binnekade Jan M, de Boer Leonie, Boszhard Laura, Paulus Frederique, Middelhoek Pauline, te Velde Anje A, Vandenbroucke-Grauls Christina M J E, Schultz Marcus J, Zaat Sebastian A J
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, The Netherlands.
Clin Infect Dis. 2008 Jun 1;46(11):1677-82. doi: 10.1086/587892.
Antibiotic resistance among microbes urgently necessitates the development of novel antimicrobial agents. Since ancient times, honey has been used successfully for treatment of infected wounds, because of its antibacterial activity. However, large variations in the in vitro antibacterial activity of various honeys have been reported and hamper its acceptance in modern medicine.
We assessed the in vitro bactericidal activity of Revamil (Bfactory), a medical-grade honey produced under controlled conditions, and assessed its efficacy for reduction of forearm skin colonization in healthy volunteers in a within-subject-controlled trial.
With Bacillus subtilis as a test strain, we demonstrated that the variation in bactericidal activity of 11 batches of medical-grade honey was <2-fold. Antibiotic-susceptible and -resistant isolates of Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, and Klebsiella oxytoca were killed within 24 h by 10%-40% (vol/vol) honey. After 2 days of application of honey, the extent of forearm skin colonization in healthy volunteers was reduced 100-fold (P < .001), and the numbers of positive skin cultures were reduced by 76% (P < .001).
Revamil is a promising topical antimicrobial agent for prevention or treatment of infections, including those caused by multidrug-resistant bacteria.
微生物中的抗生素耐药性迫切需要开发新型抗菌剂。自古以来，蜂蜜因其抗菌活性而成功用于治疗感染伤口。然而，已有报道称不同蜂蜜的体外抗菌活性存在很大差异，这阻碍了其在现代医学中的应用。
我们评估了Revamil（Bfactory）的体外杀菌活性，Revamil是一种在受控条件下生产的医用级蜂蜜，并在一项受试者自身对照试验中评估了其对健康志愿者前臂皮肤定植的减少效果。
以枯草芽孢杆菌为测试菌株，我们证明11批医用级蜂蜜的杀菌活性差异小于2倍。金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌、大肠杆菌、铜绿假单胞菌、阴沟肠杆菌和产酸克雷伯菌的抗生素敏感和耐药菌株在24小时内被10% - 40%（体积/体积）的蜂蜜杀死。在涂抹蜂蜜2天后，健康志愿者前臂皮肤定植程度降低了100倍（P <.001），皮肤培养阳性数量减少了76%（P <.001）。
Revamil是一种有前景的局部抗菌剂，可用于预防或治疗感染，包括由多重耐药菌引起的感染。
