Tesh R, Saidi S, Javadian E, Loh P, Nadim A
Am J Trop Med Hyg. 1977 Mar;26(2):299-306. doi: 10.4269/ajtmh.1977.26.299.
The characteristics and ecology of Isfahan virus, a new vesicular stomatitis virus (VSV) serotype, are described. Two strains of the agent were isolated from pools of Phlebotomus papatasi collected in Iran in 1975. Its animal pathogenicity, growth rate, cytopathic effect, and plaque morphology are similar to those of the other VSV serotypes. Electron microscopic examination of the virus demonstrated a bullet shape, the presence of truncated particles and maturation at plasma membranes. Antigenic relationships between Isfahan virus and three other VSV serotypes (Cocal, Piry, and Chandipura) were demonstrated by complement fixation or neutralization tests. A high prevalence of Isfahan neutralizing antibodies was found in human sera from several regions of Iran, suggesting that the virus may be of some public health importance. All of the residents over 5 years of age in the village where the virus was isolated had been infected. Neutralizing antibodies to Isfahan virus were also found in sera of Iranian gerbils but not in domestic animals. Results of this study suggest that the ecology of Isfahan virus is distinct from the other VSV serotypes and involves chiefly humans, gerbils, and sandflies, a pattern also observed with cutaneous leishmaniasis and sandfly fever in Iran.
本文描述了一种新的水疱性口炎病毒（VSV）血清型——伊斯法罕病毒的特征及生态学特性。1975年在伊朗采集的巴氏白蛉群体中分离出了该病原体的两个毒株。其动物致病性、生长速率、细胞病变效应及蚀斑形态与其他VSV血清型相似。病毒的电子显微镜检查显示其呈子弹状，存在截短颗粒且在细胞膜处成熟。通过补体结合或中和试验证明了伊斯法罕病毒与其他三种VSV血清型（科卡尔、皮里和钱迪普拉）之间的抗原关系。在伊朗多个地区的人类血清中发现伊斯法罕中和抗体的高流行率，这表明该病毒可能具有一定的公共卫生重要性。分离出该病毒的村庄中所有5岁以上居民均已感染。在伊朗沙土鼠血清中也发现了针对伊斯法罕病毒的中和抗体，但在家畜血清中未发现。本研究结果表明，伊斯法罕病毒的生态学特性与其他VSV血清型不同，主要涉及人类、沙土鼠和白蛉，伊朗的皮肤利什曼病和白蛉热也观察到了这种模式。
