天然存在的人源单克隆抗体可中和 1918 年和 2009 年大流行的甲型流感（H1N1）病毒。
Naturally occurring human monoclonal antibodies neutralize both 1918 and 2009 pandemic influenza A (H1N1) viruses.
机构信息
Departments of Pediatrics and of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.
出版信息
J Virol. 2010 Mar;84(6):3127-30. doi: 10.1128/JVI.02184-09. Epub 2009 Dec 30.
Abstract
The 2009 pandemic influenza A (H1N1) virus exhibits hemagglutinin protein sequence homology with the 1918 pandemic influenza virus. We found that human monoclonal antibodies recognized the Sa antigenic site on the head domains of both 1918 and 2009 hemagglutinins, a site that is hypervariable due to immune selection. These antibodies exhibited high potency against the 2009 virus in vitro, and one exerted a marked therapeutic effect in vivo.
摘要
2009 年甲型 H1N1 流感病毒的血凝素蛋白与 1918 年流感大流行病毒具有序列同源性。我们发现，人源单克隆抗体识别 1918 年和 2009 年血凝素头部结构域上的 Sa 抗原表位，该表位由于免疫选择而高度变异。这些抗体在体外对 2009 病毒具有高效价，其中一种抗体在体内表现出显著的治疗效果。
相似文献
1
Naturally occurring human monoclonal antibodies neutralize both 1918 and 2009 pandemic influenza A (H1N1) viruses.天然存在的人源单克隆抗体可中和 1918 年和 2009 年大流行的甲型流感（H1N1）病毒。
J Virol. 2010 Mar;84(6):3127-30. doi: 10.1128/JVI.02184-09. Epub 2009 Dec 30.
2
Mapping of a Novel H3-Specific Broadly Neutralizing Monoclonal Antibody Targeting the Hemagglutinin Globular Head Isolated from an Elite Influenza Virus-Immunized Donor Exhibiting Serological Breadth.新型 H3 特异性广谱中和单克隆抗体的鉴定 该抗体靶向血凝素球状头部，来源于一位具有广泛血清学反应性的流感病毒免疫供者
J Virol. 2020 Feb 28;94(6). doi: 10.1128/JVI.01035-19.
3
Potential Role of Nonneutralizing IgA Antibodies in Cross-Protective Immunity against Influenza A Viruses of Multiple Hemagglutinin Subtypes.非中和性 IgA 抗体在针对多种血凝素亚型流感 A 病毒的交叉保护免疫中的潜在作用。
J Virol. 2020 Jun 1;94(12). doi: 10.1128/JVI.00408-20.
4
Escape mutants of pandemic influenza A/H1N1 2009 virus: variations in antigenic specificity and receptor affinity of the hemagglutinin.大流行性流感 A/H1N1 2009 病毒的逃逸突变体：血凝素的抗原特异性和受体亲和力的变化。
Virus Res. 2012 Jun;166(1-2):61-7. doi: 10.1016/j.virusres.2012.03.003. Epub 2012 Mar 21.
5
Immune Escape Adaptive Mutations in Hemagglutinin Are Responsible for the Antigenic Drift of Eurasian Avian-Like H1N1 Swine Influenza Viruses.免疫逃逸适应性突变使欧亚类禽 H1N1 猪流感病毒发生抗原漂移。
J Virol. 2022 Aug 24;96(16):e0097122. doi: 10.1128/jvi.00971-22. Epub 2022 Aug 2.
6
7
A highly conserved neutralizing epitope on group 2 influenza A viruses.群组 2 流感病毒上一个高度保守的中和表位。
Science. 2011 Aug 12;333(6044):843-50. doi: 10.1126/science.1204839. Epub 2011 Jul 7.
8
Antibodies against swine influenza virus neutralize the pandemic influenza virus A/H1N1.抗猪流感病毒抗体可中和甲型 H1N1 大流行流感病毒。
Mol Med Rep. 2011 Mar-Apr;4(2):209-14. doi: 10.3892/mmr.2011.410. Epub 2011 Jan 3.
9
Structural basis of preexisting immunity to the 2009 H1N1 pandemic influenza virus.对 2009 年 H1N1 大流行流感病毒预先存在免疫的结构基础。
Science. 2010 Apr 16;328(5976):357-60. doi: 10.1126/science.1186430. Epub 2010 Mar 25.
10
Highly conserved sequences for human neutralization epitope on hemagglutinin of influenza A viruses H3N2, H1N1 and H5N1: Implication for human monoclonal antibody recognition.高度保守的人甲型流感病毒 H3N2、H1N1 和 H5N1 血凝素中和表位序列：对人源单克隆抗体识别的意义。
Biochem Biophys Res Commun. 2010 Mar 19;393(4):614-8. doi: 10.1016/j.bbrc.2010.02.031. Epub 2010 Feb 10.
引用本文的文献
1
De novo design of D-peptide ligands: Application to influenza virus hemagglutinin.D-肽配体的从头设计：在流感病毒血凝素中的应用。
Proc Natl Acad Sci U S A. 2025 Jul;122(26):e2426554122. doi: 10.1073/pnas.2426554122. Epub 2025 Jun 27.
2
Severe Bacterial Superinfection of Influenza Pneumonia in Immunocompetent Young Patients: Case Reports.免疫功能正常的年轻患者流感肺炎的严重细菌重叠感染：病例报告
J Clin Med. 2024 Sep 24;13(19):5665. doi: 10.3390/jcm13195665.
3
Antigen spacing on protein nanoparticles influences antibody responses to vaccination.蛋白质纳米颗粒上的抗原间距影响疫苗接种后的抗体反应。
Cell Rep. 2023 Dec 26;42(12):113552. doi: 10.1016/j.celrep.2023.113552. Epub 2023 Dec 13.
4
Antigen spacing on protein nanoparticles influences antibody responses to vaccination.蛋白质纳米颗粒上的抗原间距会影响疫苗接种的抗体反应。
bioRxiv. 2023 May 24:2023.05.23.541980. doi: 10.1101/2023.05.23.541980.
5
Low quality antibody responses in critically ill patients hospitalized with pandemic influenza A(H1N1)pdm09 virus infection.危重症患者在感染大流行流感 A(H1N1)pdm09 病毒住院期间抗体反应不佳。
Sci Rep. 2022 Sep 2;12(1):14971. doi: 10.1038/s41598-022-18977-0.
6
Identification of a cross-neutralizing antibody that targets the receptor binding site of H1N1 and H5N1 influenza viruses.鉴定一种针对 H1N1 和 H5N1 流感病毒受体结合位点的具有交叉中和作用的抗体。
Nat Commun. 2022 Sep 2;13(1):5182. doi: 10.1038/s41467-022-32926-5.
7
Implementing sequence-based antigenic distance calculation into immunological shape space model.将基于序列的抗原距离计算方法引入免疫形状空间模型。
BMC Bioinformatics. 2020 Jun 19;21(1):256. doi: 10.1186/s12859-020-03594-3.
8
Pathogenesis of pandemic H1N1 2009 influenza virus infection and the implication on management.2009年甲型H1N1流感大流行病毒感染的发病机制及其对治疗的启示。
Front Med China. 2010;4(2):147-156. doi: 10.1007/s11684-010-0030-9. Epub 2010 Apr 28.
9
Human Susceptibility to Influenza Infection and Severe Disease.人类对流感感染和严重疾病的易感性。
Cold Spring Harb Perspect Med. 2021 May 3;11(5):a038711. doi: 10.1101/cshperspect.a038711.
10
Assessment of the influenza vaccine (VAXIGRIP) in triggering a humoural immune response.评估流感疫苗（VAXIGRIP）引发体液免疫反应的情况。
J Taibah Univ Med Sci. 2017 Feb 20;12(6):523-527. doi: 10.1016/j.jtumed.2017.01.001. eCollection 2017 Dec.
本文引用的文献
1
SWINE INFLUENZA : I. EXPERIMENTAL TRANSMISSION AND PATHOLOGY.猪流感：I. 实验传播与病理学。
J Exp Med. 1931 Jul 31;54(3):349-59. doi: 10.1084/jem.54.3.349.
2
Cross-reactive antibody responses to the 2009 pandemic H1N1 influenza virus.对2009年甲型H1N1流感大流行病毒的交叉反应性抗体应答。
N Engl J Med. 2009 Nov 12;361(20):1945-52. doi: 10.1056/NEJMoa0906453. Epub 2009 Sep 10.
3
In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses.新型猪源H1N1流感病毒的体外和体内特性研究
Nature. 2009 Aug 20;460(7258):1021-5. doi: 10.1038/nature08260.
4
Transmission and pathogenesis of swine-origin 2009 A(H1N1) influenza viruses in ferrets and mice.猪源2009甲型（H1N1）流感病毒在雪貂和小鼠中的传播与发病机制
Science. 2009 Jul 24;325(5939):484-7. doi: 10.1126/science.1177238. Epub 2009 Jul 2.
5
Outbreak of swine-origin influenza A (H1N1) virus infection - Mexico, March-April 2009.2009年3月至4月墨西哥甲型H1N1流感病毒感染疫情
MMWR Morb Mortal Wkly Rep. 2009 May 8;58(17):467-70.
6
Cross-protective potential of a novel monoclonal antibody directed against antigenic site B of the hemagglutinin of influenza A viruses.一种针对甲型流感病毒血凝素抗原位点B的新型单克隆抗体的交叉保护潜力。
PLoS Pathog. 2009 Mar;5(3):e1000350. doi: 10.1371/journal.ppat.1000350. Epub 2009 Mar 20.
7
Antibody recognition of a highly conserved influenza virus epitope.对高度保守的流感病毒表位的抗体识别。
Science. 2009 Apr 10;324(5924):246-51. doi: 10.1126/science.1171491. Epub 2009 Feb 26.
8
Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses.甲型禽流感病毒和人流感病毒广谱中和的结构与功能基础
Nat Struct Mol Biol. 2009 Mar;16(3):265-73. doi: 10.1038/nsmb.1566. Epub 2009 Feb 22.
9
Neutralizing antibodies derived from the B cells of 1918 influenza pandemic survivors.源自1918年流感大流行幸存者B细胞的中和抗体。
Nature. 2008 Sep 25;455(7212):532-6. doi: 10.1038/nature07231. Epub 2008 Aug 17.
10
Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States.对来自美国的1型血凝素亚型猪流感病毒的评估。
Vet Microbiol. 2006 Dec 20;118(3-4):212-22. doi: 10.1016/j.vetmic.2006.07.017. Epub 2006 Aug 1.
Zotero 插件