De Ferrari M, Artuso M, Bonassi S, Bonatti S, Cavalieri Z, Pescatore D, Marchini E, Pisano V, Abbondandolo A
Laboratory of Mutagenesis, IST, Genoa, Italy.
Mutat Res. 1991 May;260(1):105-13. doi: 10.1016/0165-1218(91)90086-2.
Chromosome aberrations (CA) and sister-chromatid exchanges (SCE) were measured in lymphocytes of (A) 32 healthy individuals working in the flower industry and exposed to pesticides, (B) 32 individuals exposed as above and hospitalized for bladder cancer, and (C) 31 controls. Compounds to which floriculturists were exposed included 18 nitro-organic herbicides and fungicides, 9 nitro-organic fungicides, 12 organophosphate and organothiophosphate insecticides, 4 hydrocarbon derivative herbicides and 5 inorganic fungicides and insecticides. 150 and 70 metaphases per individual were scored for CA and SCE, respectively. A significant increase in the incidence of CA and SCE was observed in both exposed groups. Cancer patients showed the presence of rare rearrangements (dicentrics, rings and quadriradials) that were not observed in controls and were present at a lower frequency in healthy exposed people. Hyperdiploid and polyploid metaphases were also significantly increased in the 2 exposed groups compared to controls. Stratifying for age or smoking habits, although affecting the significance of individual data, did not change the substance of the results.
对以下三组人群的淋巴细胞进行了染色体畸变（CA）和姐妹染色单体交换（SCE）检测：（A）32名从事花卉行业且接触农药的健康个体；（B）32名上述接触农药且因膀胱癌住院的个体；（C）31名对照者。花卉种植者接触的化合物包括18种硝基有机除草剂和杀菌剂、9种硝基有机杀菌剂、12种有机磷和有机硫代磷酸酯杀虫剂、4种烃类衍生物除草剂以及5种无机杀菌剂和杀虫剂。分别对每位个体的150个和70个中期分裂相进行CA和SCE评分。在两个接触组中均观察到CA和SCE发生率显著增加。癌症患者出现了罕见的重排（双着丝粒、环状和四射体），对照组未观察到这些情况，且在健康接触者中出现频率较低。与对照组相比，两个接触组中的超二倍体和多倍体中期分裂相也显著增加。按年龄或吸烟习惯分层，虽然会影响个体数据的显著性，但并未改变结果的实质。
