Accili D, Taylor S I
Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4708-12. doi: 10.1073/pnas.88.11.4708.
To study the role of the insulin receptor in determining adipocyte differentiation of the mouse cell line 3T3-L1, we have introduced a mutation that inactivates the insulin receptor gene by homologous recombination. In two independent clones, inactivation of one allele of the insulin receptor gene was associated with a 50-70% reduction in the number of insulin receptors. In addition, both clones were markedly impaired in their ability to differentiate into adipocytes. The defect in adipocyte-specific differentiation was corrected by expression of transfected human insulin receptor cDNA. These data suggest that the insulin receptor may play an important role in promoting differentiation of 3T3-L1 cells into adipocytes in vitro.
为了研究胰岛素受体在决定小鼠细胞系3T3-L1脂肪细胞分化中的作用，我们通过同源重组引入了一个使胰岛素受体基因失活的突变。在两个独立的克隆中，胰岛素受体基因一个等位基因的失活与胰岛素受体数量减少50 - 70%相关。此外，两个克隆在分化为脂肪细胞的能力上均明显受损。转染的人胰岛素受体cDNA的表达纠正了脂肪细胞特异性分化的缺陷。这些数据表明，胰岛素受体可能在体外促进3T3-L1细胞向脂肪细胞分化中起重要作用。
