Sousa Erlânio O, Silva Natálya F, Rodrigues Fabiola F G, Campos Adriana R, Lima Sidney G, Costa José Galberto M
Programa de Pós-Graduação em Bioprospecção Molecular, Departamento de Química Biológica, Laboratório de Pesquisa de Produtos Naturais, Universidade Regional do Cariri, Rua Cel. Antônio Luiz 1161, Pimenta, 63105-000, Crato-CE, Brazil.
Pharmacogn Mag. 2010 Apr;6(22):79-82. doi: 10.4103/0973-1296.62890. Epub 2010 May 5.
In this work, the chemical constituents, antibacterial and modulatory activities of the essential oil of Lantana camara Linn were studied. The essential oil was extracted from the leaves of L. camara by hydrodistillation method using Clevenger's apparatus and its chemical constituents were separated and identified by GC-MS, and the relative content of each constituent was determined by area normalization. Among the 25 identified components, bicyclogermacrene (19.42%), isocaryophyllene (16.70%), valecene (12.94%) and germacrene D (12.34%) were the main constituents. The oil was examined to antibacterial and modulatory activities against the multiresistant strains of Escherichia coli and Staphylococcus aureus by microdilution test. The results show an inhibitory activity to E. coli (MIC 512 mug/ml) and S. aureus (MIC 256 mug/ml). The synergism of the essential oil and aminoglycosides was verified too, with significant reduction of MICs (7 x, 1250-5 mug/ml) against E. coli. It is suggested that the essential oil of Lantana camara Linn could be used as a source of plant-derived natural products with resistance-modifying activity.
本研究对马缨丹（Lantana camara Linn）精油的化学成分、抗菌及调节活性进行了研究。采用克莱文杰装置通过水蒸馏法从马缨丹叶片中提取精油，并用气相色谱 - 质谱联用仪（GC-MS）对其化学成分进行分离和鉴定，通过面积归一化法测定各成分的相对含量。在鉴定出的25种成分中，双环吉玛烯（19.42%）、异石竹烯（16.70%）、瓦伦烯（12.94%）和吉玛烯D（12.34%）为主要成分。通过微量稀释试验检测该精油对多重耐药性大肠杆菌和金黄色葡萄球菌的抗菌及调节活性。结果表明，该精油对大肠杆菌（MIC 512μg/ml）和金黄色葡萄球菌（MIC 256μg/ml）具有抑制活性。同时也验证了该精油与氨基糖苷类药物的协同作用，对大肠杆菌的MIC显著降低（7倍，1250 - 5μg/ml）。提示马缨丹精油可作为具有耐药性调节活性的植物源天然产物来源。
