日本实验舱“希望号”中的秀丽隐杆线虫RNA干扰空间实验（CERISE）。
C. elegans RNAi space experiment (CERISE) in Japanese Experiment Module KIBO.
作者信息
Higashitani Atsushi, Hashizume Toko, Sugimoto Tomoko, Mori Chihiro, Nemoto Kanako, Etheridge Timothy, Higashitani Nahoko, Takanami Takako, Suzuki Hiromi, Fukui Keiji, Yamazaki Takashi, Ishioka Noriaki, Szewczyk Nathaniel, Higashibata Akira
机构信息
Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577, Japan.
出版信息
Biol Sci Space. 2009 Oct 1;23(4):183-187. doi: 10.2187/bss.23.183.
Abstract
We have started a space experiment using an experimental organism, the nematode Caenorhabditis elegans, in the Japanese Experiment Module, KIBO, of the International Space Station (ISS). The specimens were boarded by space shuttle Atlantis on mission STS-129 which launched from NASA Kennedy Space Center on November 16, 2009. The purpose of the experiment was several-fold: (i) to verify the efficacy of RNA interference (RNAi) in space, (ii) to monitor transcriptional and post-translational alterations in the entire genome in space, and (iii) to investigate mechanisms regulating and countermeasures for muscle alterations in response to the space environment. In particular, this will be the first study to utilize RNAi in space.
摘要
我们已利用实验生物秀丽隐杆线虫在国际空间站（ISS）的日本实验舱“希望”号中启动了一项太空实验。这些样本由执行STS - 129任务的亚特兰蒂斯号航天飞机搭载，该任务于2009年11月16日从美国国家航空航天局肯尼迪航天中心发射。该实验目的有多个方面：（i）验证RNA干扰（RNAi）在太空中的有效性；（ii）监测太空中整个基因组的转录和翻译后变化；（iii）研究应对太空环境引起的肌肉变化的调节机制和对策。特别是，这将是首次在太空中利用RNAi的研究。
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Early Hum Dev. 2009 Oct;85(10 Suppl):S31-5. doi: 10.1016/j.earlhumdev.2009.08.013. Epub 2009 Oct 14.
2
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3
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Adv Space Res. 2008;41(5):807-815. doi: 10.1016/j.asr.2007.11.015.
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Potentiality of small interfering RNAs (siRNA) as recent therapeutic targets for gene-silencing.小干扰RNA（siRNA）作为基因沉默近期治疗靶点的潜力。
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