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与腹泻粪便中人类轮状病毒相关的RNA聚合酶。

RNA polymerase associated with human rotaviruses in diarrhea stools.

作者信息

Hruska J F, Notter M F, Menegus M A, Steinhoff M C

出版信息

J Virol. 1978 May;26(2):544-6. doi: 10.1128/JVI.26.2.544-546.1978.

Abstract

RNA polymerase activity was detected in six stools which were partially purified by high-speed centrifugation from infants with rotavirus gastroenteritis, but was not detected in five stools which were negative for rotavirus by counterimmunoelectrophoresis and radioimmunoassay. The polymerase activity was associated with the 1.38-g/ml rotavirus band after purification in a CsCl gradient.

摘要

在通过高速离心从轮状病毒肠胃炎婴儿中部分纯化的6份粪便中检测到了RNA聚合酶活性,但在通过对流免疫电泳和放射免疫测定法检测轮状病毒呈阴性的5份粪便中未检测到该活性。纯化后,在氯化铯梯度中,聚合酶活性与1.38 g/ml的轮状病毒条带相关。

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