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与腹泻粪便中人类轮状病毒相关的RNA聚合酶。

RNA polymerase associated with human rotaviruses in diarrhea stools.

作者信息

Hruska J F, Notter M F, Menegus M A, Steinhoff M C

出版信息

J Virol. 1978 May;26(2):544-6. doi: 10.1128/JVI.26.2.544-546.1978.

DOI:10.1128/JVI.26.2.544-546.1978
PMID:207902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354093/
Abstract

RNA polymerase activity was detected in six stools which were partially purified by high-speed centrifugation from infants with rotavirus gastroenteritis, but was not detected in five stools which were negative for rotavirus by counterimmunoelectrophoresis and radioimmunoassay. The polymerase activity was associated with the 1.38-g/ml rotavirus band after purification in a CsCl gradient.

摘要

在通过高速离心从轮状病毒肠胃炎婴儿中部分纯化的6份粪便中检测到了RNA聚合酶活性,但在通过对流免疫电泳和放射免疫测定法检测轮状病毒呈阴性的5份粪便中未检测到该活性。纯化后,在氯化铯梯度中,聚合酶活性与1.38 g/ml的轮状病毒条带相关。

相似文献

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RNA polymerase associated with human rotaviruses in diarrhea stools.与腹泻粪便中人类轮状病毒相关的RNA聚合酶。
J Virol. 1978 May;26(2):544-6. doi: 10.1128/JVI.26.2.544-546.1978.
2
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Bol Med Hosp Infant Mex. 1978 Mar-Apr;35(2):217-22.
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Routine diagnosis of human rotaviruses in stools.粪便中人类轮状病毒的常规诊断。
J Clin Pathol. 1978 Feb;31(2):175-8. doi: 10.1136/jcp.31.2.175.
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A study of the prevalence of rotavirus infection in children with gastroenteritis admitted to an infectious diseases hospital.对一家传染病医院收治的患肠胃炎儿童轮状病毒感染患病率的研究。
J Med Virol. 1977;1(1):69-77. doi: 10.1002/jmv.1890010109.
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Simplified radioimmunoassay for detection of human rotavirus in stools.用于检测粪便中人类轮状病毒的简易放射免疫测定法。
J Infect Dis. 1978 Dec;138(6):906-10. doi: 10.1093/infdis/138.6.906.
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Characterization of RNA polymerase products of Nebraska calf diarrhea virus and SA11 rotavirus.内布拉斯加犊牛腹泻病毒和SA11轮状病毒RNA聚合酶产物的特性分析
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Comparative study on the mechanisms of rotavirus inactivation by sodium dodecyl sulfate and ethylenediaminetetraacetate.十二烷基硫酸钠和乙二胺四乙酸对轮状病毒灭活机制的比较研究
Appl Environ Microbiol. 1980 Jun;39(6):1148-53. doi: 10.1128/aem.39.6.1148-1153.1980.
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8
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本文引用的文献

1
Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests.粪便中的呼肠孤病毒样病原体:与婴儿腹泻的关联及血清学检测的发展
Science. 1974 Sep 20;185(4156):1049-53. doi: 10.1126/science.185.4156.1049.
2
Isolation and characterization of two types of bluetongue virus particles.两种蓝舌病毒颗粒的分离与鉴定
Virology. 1972 Nov;50(2):495-506. doi: 10.1016/0042-6822(72)90400-x.
3
RNA polymerase activity in purified reoviruses.纯化呼肠孤病毒中的RNA聚合酶活性
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462-9. doi: 10.1073/pnas.61.4.1462.
4
Letter: Virus particles in gastroenteritis.信函:肠胃炎中的病毒颗粒。
Lancet. 1973 Dec 29;2(7844):1497. doi: 10.1016/s0140-6736(73)92760-8.
5
Solid-phase radioimmunoassay for the detection of rotavirus.用于检测轮状病毒的固相放射免疫测定法。
Infect Immun. 1977 May;16(2):439-44. doi: 10.1128/iai.16.2.439-444.1977.
6
Purification and preliminary characterization of infantile gastroenteritis virus (orbivirus group).婴儿肠胃炎病毒(环状病毒属)的纯化及初步特性分析
Intervirology. 1975;5(3-4):233-8. doi: 10.1159/000149919.
7
Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses.对人、牛和猴轮状病毒进行进一步的生化特性鉴定,包括表面糖蛋白的检测。
J Virol. 1977 Oct;24(1):91-8. doi: 10.1128/JVI.24.1.91-98.1977.
8
Characterization of two particle types of calf rotavirus.
J Gen Virol. 1976 May;31(2):245-50. doi: 10.1099/0022-1317-31-2-245.
9
Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin.人类和小牛源腹泻病毒的生化及生物物理特性
J Virol. 1975 Nov;16(5):1229-35. doi: 10.1128/JVI.16.5.1229-1235.1975.
10
Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization.通过补体结合试验和中和试验研究不同物种轮状病毒之间的血清学关系。
Arch Virol. 1977;53(4):287-94. doi: 10.1007/BF01315627.