Department of Life Sciences, National Central University, Taoyuan, Taiwan.
J Sci Food Agric. 2011 Jan 15;91(1):134-41. doi: 10.1002/jsfa.4163. Epub 2010 Sep 7.
Atherosclerosis and restenosis are inflammatory responses involving free radicals and lipid peroxidation and may be prevented/cured by antioxidant-mediated lipid peroxidation inhibition. Salvianolic acid (Sal B), a water-soluble antioxidant obtained from a Chinese medicinal herb, is believed to have multiple preventive and therapeutic effects against human vascular diseases. In this study the in vitro and in vivo inhibitory effects of Sal B on oxidative stress were determined.
In human aortic endothelial cells (HAECs), Sal B reduced oxidative stress, inhibited low-density lipoprotein (LDL) oxidation and reduced oxidised LDL-induced cytotoxicity. Sal B inhibited Cu(2+) -induced LDL oxidation in vitro (with a potency 16.3 times that of probucol) and attenuated HAEC-mediated LDL oxidation as well as reactive oxygen species (ROS) production. In cholesterol-fed New Zealand White rabbits (with probucol as positive control), Sal B intake reduced Cu(2+) -induced LDL oxidation, lipid deposition in the thoracic aorta, intimal thickness of the aortic arch and thoracic aorta and neointimal formation in the abdominal aorta.
The data obtained in this study suggest that Sal B protects HAECs from oxidative injury-mediated cell death via inhibition of ROS production. The antioxidant activity of Sal B may help explain its efficacy in the treatment of vascular diseases.
动脉粥样硬化和再狭窄是涉及自由基和脂质过氧化的炎症反应，可通过抗氧化剂介导的脂质过氧化抑制来预防/治疗。丹酚酸 B（Sal B）是一种从中药中提取的水溶性抗氧化剂，被认为对人类血管疾病具有多种预防和治疗作用。在这项研究中，测定了 Sal B 对氧化应激的体外和体内抑制作用。
在人主动脉内皮细胞（HAEC）中，Sal B 减轻氧化应激，抑制低密度脂蛋白（LDL）氧化，并降低氧化 LDL 诱导的细胞毒性。Sal B 抑制体外 Cu（2+）诱导的 LDL 氧化（效力是普罗布考的 16.3 倍），并减弱 HAEC 介导的 LDL 氧化和活性氧（ROS）产生。在胆固醇喂养的新西兰白兔（以普罗布考为阳性对照）中，Sal B 摄入可降低 Cu（2+）诱导的 LDL 氧化、胸主动脉中的脂质沉积、主动脉弓和胸主动脉的内膜厚度以及腹主动脉的新生内膜形成。
本研究获得的数据表明，Sal B 通过抑制 ROS 产生来保护 HAEC 免受氧化损伤介导的细胞死亡。Sal B 的抗氧化活性可能有助于解释其在治疗血管疾病中的功效。
