Harris S J, Cecil H C, Bitman J
Environ Health Perspect. 1978 Apr;23:295-300. doi: 10.1289/ehp.7823295.
Pregnant Sprague Dawley rats were given 0, 0.25, 0.5, 1, 5, and 10 mg of a commercial polybrominated biphenyl, FireMaster BP-6 (PBB), in olive oil by gavage each day from days 7 through 15 of pregnancy. Laboratory chow and water were given ad libitum. Treatment with PBB had no significant effect on body weight gain, food and water consumption, and urine production. The mothers were killed on day 20, and the only significant effect observed was an increased liver weight of those given 1, 5, and 10 mg PBB. Spleen, kidney, ovarian, gravid uterine, and perirenal fat pad weights were similar to those of control mothers. PBB had no significant effect on number of live/dead fetuses, crown-rump length or fetal weight. No grossly malformed fetuses were observed in PBB-treated mothers. The effects of PBB transfer from mothers to nursing pups was studied by reciprocal exchange of pups between control mothers and mothers treated with 10 mg PBB. When the pups were 21 days old, they were weaned and fed control chow. The following four combinations of prenatal-postnatal exposure were studied: control-control (C:C); control-PBB (C:PBB); PBB-control (PBB:C); and PBB-PBB. Although the birth weights of pups from PBB-treated mothers were similar to those of the controls, body weights of 60-day-old males exposed prenatally and postnatally (PBB:C, C:PBB, and PBB:PBB) were less (p less than 0.05) than those of the controls (C:C). The weights of the perirenal fat pads of male and female pups exposed to PBB were less (p less than 0.05) than the control. Liver weights, on a body weight basis, were higher in male and female pups exposed to PBB. Vaginal openings were delayed; the percentages of 36-day-old pups with open vaginas were 50 (C:C), 38 (PBB:C), 28 (C:PBB), and 30 (PBB:PBB).
从怀孕第7天至第15天，每天通过灌胃法给怀孕的斯普拉格-道利大鼠喂食0、0.25、0.5、1、5和10毫克市售多溴联苯FireMaster BP - 6（PBB），溶剂为橄榄油。实验室饲料和水可随意取用。PBB处理对体重增加、食物和水的消耗以及尿量均无显著影响。在第20天处死母鼠，观察到的唯一显著影响是给予1、5和10毫克PBB的母鼠肝脏重量增加。脾脏、肾脏、卵巢、妊娠子宫和肾周脂肪垫的重量与对照母鼠相似。PBB对活胎/死胎数量、顶臀长度或胎儿体重均无显著影响。在接受PBB处理的母鼠中未观察到严重畸形的胎儿。通过在对照母鼠和接受10毫克PBB处理的母鼠之间相互交换幼崽，研究了PBB从母体转移至哺乳幼崽的影响。幼崽21日龄时断奶并喂食对照饲料。研究了以下四种产前 - 产后暴露组合：对照 - 对照（C:C）；对照 - PBB（C:PBB）；PBB - 对照（PBB:C）；以及PBB - PBB。虽然来自PBB处理母鼠的幼崽出生体重与对照幼崽相似，但产前和产后暴露（PBB:C、C:PBB和PBB:PBB）的60日龄雄性幼崽体重低于（p小于0.05）对照（C:C）。暴露于PBB的雄性和雌性幼崽肾周脂肪垫重量低于（p小于0.05）对照。以体重计，暴露于PBB的雄性和雌性幼崽肝脏重量较高。阴道开口延迟；36日龄阴道开放的幼崽百分比分别为50（C:C）、38（PBB:C）、28（C:PBB）和30（PBB:PBB）。
