Lu Rong-Wen, Wang Ben-Quan, Zhang Qiu-Xiang, Yao Xin-Cheng
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA ; These authors contributed equally to this work.
Biomed Opt Express. 2013 Aug 19;4(9):1673-82. doi: 10.1364/BOE.4.001673. eCollection 2013.
High resolution microscopy is essential for advanced study of biological structures and accurate diagnosis of medical diseases. The spatial resolution of conventional microscopes is light diffraction limited. Structured illumination has been extensively explored to break the diffraction limit in wide field light microscopy. However, deployable application of the structured illumination in scanning laser microscopy is challenging due to the complexity of the illumination system and possible phase errors in sequential illumination patterns required for super-resolution reconstruction. We report here a super-resolution scanning laser imaging system which employs virtually structured detection (VSD) to break the diffraction limit. Without the complexity of structured illumination, VSD provides an easy, low-cost and phase-artifact free strategy to achieve super-resolution in scanning laser microscopy.
高分辨率显微镜对于生物结构的深入研究和医学疾病的准确诊断至关重要。传统显微镜的空间分辨率受光衍射限制。结构照明已被广泛探索以突破宽视场光学显微镜中的衍射极限。然而，由于照明系统的复杂性以及超分辨率重建所需的顺序照明模式中可能存在的相位误差，结构照明在扫描激光显微镜中的可部署应用具有挑战性。我们在此报告一种超分辨率扫描激光成像系统，该系统采用虚拟结构检测（VSD）来突破衍射极限。VSD无需结构照明的复杂性，提供了一种简单、低成本且无相位伪影的策略，以在扫描激光显微镜中实现超分辨率。
