通过虚拟结构检测实现的超分辨率扫描激光显微镜技术。
Super-resolution scanning laser microscopy through virtually structured detection.
作者信息
Lu Rong-Wen, Wang Ben-Quan, Zhang Qiu-Xiang, Yao Xin-Cheng
机构信息
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA ; These authors contributed equally to this work.
出版信息
Biomed Opt Express. 2013 Aug 19;4(9):1673-82. doi: 10.1364/BOE.4.001673. eCollection 2013.
Abstract
High resolution microscopy is essential for advanced study of biological structures and accurate diagnosis of medical diseases. The spatial resolution of conventional microscopes is light diffraction limited. Structured illumination has been extensively explored to break the diffraction limit in wide field light microscopy. However, deployable application of the structured illumination in scanning laser microscopy is challenging due to the complexity of the illumination system and possible phase errors in sequential illumination patterns required for super-resolution reconstruction. We report here a super-resolution scanning laser imaging system which employs virtually structured detection (VSD) to break the diffraction limit. Without the complexity of structured illumination, VSD provides an easy, low-cost and phase-artifact free strategy to achieve super-resolution in scanning laser microscopy.
摘要
高分辨率显微镜对于生物结构的深入研究和医学疾病的准确诊断至关重要。传统显微镜的空间分辨率受光衍射限制。结构照明已被广泛探索以突破宽视场光学显微镜中的衍射极限。然而，由于照明系统的复杂性以及超分辨率重建所需的顺序照明模式中可能存在的相位误差，结构照明在扫描激光显微镜中的可部署应用具有挑战性。我们在此报告一种超分辨率扫描激光成像系统，该系统采用虚拟结构检测（VSD）来突破衍射极限。VSD无需结构照明的复杂性，提供了一种简单、低成本且无相位伪影的策略，以在扫描激光显微镜中实现超分辨率。
相似文献
1
Super-resolution scanning laser microscopy through virtually structured detection.通过虚拟结构检测实现的超分辨率扫描激光显微镜技术。
Biomed Opt Express. 2013 Aug 19;4(9):1673-82. doi: 10.1364/BOE.4.001673. eCollection 2013.
2
Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection.基于虚拟结构检测的超分辨率扫描激光显微镜
Crit Rev Biomed Eng. 2015;43(4):297-322. doi: 10.1615/CritRevBiomedEng.2016016445.
3
Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging.超分辨率眼底镜：视网膜成像中分辨率提高的虚拟结构检测。
Exp Biol Med (Maywood). 2021 Feb;246(3):249-259. doi: 10.1177/1535370220970533. Epub 2020 Nov 27.
4
Rapid super-resolution line-scanning microscopy through virtually structured detection.通过虚拟结构检测实现快速超分辨率线扫描显微镜技术。
Opt Lett. 2015 Apr 15;40(8):1683-6. doi: 10.1364/OL.40.001683.
5
Virtually structured detection enables super-resolution ophthalmoscopy of rod and cone photoreceptors in human retina.虚拟结构检测实现了对人视网膜中视杆和视锥光感受器的超分辨率检眼镜检查。
Quant Imaging Med Surg. 2021 Mar;11(3):1060-1069. doi: 10.21037/qims-20-542.
6
Recent advances in super-resolution fluorescence imaging and its applications in biology.超分辨率荧光成像技术的最新进展及其在生物学中的应用。
J Genet Genomics. 2013 Dec 20;40(12):583-95. doi: 10.1016/j.jgg.2013.11.003. Epub 2013 Nov 23.
7
Super-resolution laser scanning microscopy through spatiotemporal modulation.基于时空调制的超分辨率激光扫描显微镜
Nano Lett. 2009 Nov;9(11):3883-9. doi: 10.1021/nl902087d.
8
Structured oblique illumination microscopy for enhanced resolution imaging of non-fluorescent, coherently scattering samples.用于非荧光、相干散射样品增强分辨率成像的结构化斜照显微镜。
Biomed Opt Express. 2012 Aug 1;3(8):1841-54. doi: 10.1364/BOE.3.001841. Epub 2012 Jul 12.
9
Interference illumination of three nonzero-order beams for LCOS-based structured illumination microscopy.基于硅基液晶的结构光照明显微镜的三束非零阶光束的干涉照明。
J Microsc. 2019 Aug;275(2):97-106. doi: 10.1111/jmi.12806. Epub 2019 May 23.
10
Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.利用结构光照明显微镜对神经元结构进行超分辨率成像。
Bioengineering (Basel). 2023 Sep 13;10(9):1081. doi: 10.3390/bioengineering10091081.
引用本文的文献
1
structured illumination ophthalmoscopy demonstration on the human retina using adaptive optics.使用自适应光学技术在人视网膜上进行结构照明检眼镜演示。
Biomed Opt Express. 2025 Jun 24;16(7):2923-2944. doi: 10.1364/BOE.559670. eCollection 2025 Jul 1.
2
Review of bio-optical imaging systems with a high space-bandwidth product.具有高空间带宽积的生物光学成像系统综述。
Adv Photonics. 2021 Jul;3(4). doi: 10.1117/1.ap.3.4.044001. Epub 2021 Jun 26.
3
Resolution-enhanced OCT and expanded framework of information capacity and resolution in coherent imaging.分辨率增强光学相干断层扫描和相干成像中信息容量和分辨率的扩展框架。
Sci Rep. 2021 Oct 15;11(1):20541. doi: 10.1038/s41598-021-99889-3.
4
Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain.直接在空间域中进行结构照明显微镜的快速图像重建。
IEEE Photonics J. 2021 Feb;13(1). doi: 10.1109/JPHOT.2021.3053110. Epub 2021 Jan 20.
5
Virtually structured detection enables super-resolution ophthalmoscopy of rod and cone photoreceptors in human retina.虚拟结构检测实现了对人视网膜中视杆和视锥光感受器的超分辨率检眼镜检查。
Quant Imaging Med Surg. 2021 Mar;11(3):1060-1069. doi: 10.21037/qims-20-542.
6
Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging.超分辨率眼底镜：视网膜成像中分辨率提高的虚拟结构检测。
Exp Biol Med (Maywood). 2021 Feb;246(3):249-259. doi: 10.1177/1535370220970533. Epub 2020 Nov 27.
7
Virtual single-pixel imaging-based deconvolution method for spatial resolution improvement in wide-field fluorescence microscopy.基于虚拟单像素成像的去卷积方法用于提高宽场荧光显微镜的空间分辨率
Biomed Opt Express. 2020 Jun 9;11(7):3648-3658. doi: 10.1364/BOE.396336. eCollection 2020 Jul 1.
8
Structured illumination imaging without grating rotation based on mirror operation on 1D Fourier spectrum.基于一维傅里叶频谱镜操作的无光栅旋转结构光照明显微成像
Opt Express. 2019 Feb 4;27(3):2016-2028. doi: 10.1364/OE.27.002016.
9
Connectomics of synaptic microcircuits: lessons from the outer retina.突触微电路的连接组学：外视网膜的启示。
J Physiol. 2017 Aug 15;595(16):5517-5524. doi: 10.1113/JP273671. Epub 2017 May 4.
10
In vivo super-resolution retinal imaging through virtually structured detection.通过虚拟结构化检测实现体内超分辨率视网膜成像。
J Biomed Opt. 2016 Dec 1;21(12):120502. doi: 10.1117/1.JBO.21.12.120502.
本文引用的文献
1
In vivo confocal intrinsic optical signal identification of localized retinal dysfunction.体内共聚焦固有光学信号定位视网膜功能障碍。
Invest Ophthalmol Vis Sci. 2012 Dec 13;53(13):8139-45. doi: 10.1167/iovs.12-10732.
2
The use of forward scatter to improve retinal vascular imaging with an adaptive optics scanning laser ophthalmoscope.利用前向散射通过自适应光学扫描激光检眼镜改善视网膜血管成像。
Biomed Opt Express. 2012 Oct 1;3(10):2537-49. doi: 10.1364/BOE.3.002537. Epub 2012 Sep 13.
3
Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes.利用光致变色膜探针对活细胞内细胞器进行超分辨率荧光成像。
Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):13978-83. doi: 10.1073/pnas.1201882109. Epub 2012 Aug 13.
4
Nanoscopy in a living mouse brain.在活体老鼠大脑中进行纳米显微镜观察。
Science. 2012 Feb 3;335(6068):551. doi: 10.1126/science.1215369.
5
In vivo confocal imaging of fast intrinsic optical signals correlated with frog retinal activation.体内共聚焦成像快速固有光学信号与青蛙视网膜激活相关联。
Opt Lett. 2011 Dec 1;36(23):4692-4. doi: 10.1364/OL.36.004692.
6
Observation of cone and rod photoreceptors in normal subjects and patients using a new generation adaptive optics scanning laser ophthalmoscope.使用新一代自适应光学扫描激光检眼镜对正常受试者和患者的视锥和视杆光感受器进行观察。
Biomed Opt Express. 2011 Aug 1;2(8):2189-201. doi: 10.1364/BOE.2.002189. Epub 2011 Jul 8.
7
Noninvasive imaging of the human rod photoreceptor mosaic using a confocal adaptive optics scanning ophthalmoscope.使用共焦自适应光学扫描检眼镜对人类视杆光感受器镶嵌进行无创成像。
Biomed Opt Express. 2011 Jul 1;2(7):1864-76. doi: 10.1364/BOE.2.001864. Epub 2011 Jun 8.
8
Integrated adaptive optics optical coherence tomography and adaptive optics scanning laser ophthalmoscope system for simultaneous cellular resolution in vivo retinal imaging.用于体内视网膜成像同时实现细胞分辨率的集成自适应光学光学相干断层扫描和自适应光学扫描激光眼科显微镜系统。
Biomed Opt Express. 2011 Jun 1;2(6):1674-86. doi: 10.1364/BOE.2.001674. Epub 2011 May 24.
9
Fast, three-dimensional super-resolution imaging of live cells.快速、三维活细胞超分辨率成像。
Nat Methods. 2011 Jun;8(6):499-508. doi: 10.1038/nmeth.1605. Epub 2011 May 8.
10
Microlens array recording of localized retinal responses.微透镜阵列记录局部视网膜反应。
Opt Lett. 2010 Nov 15;35(22):3838-40. doi: 10.1364/OL.35.003838.
Zotero 插件