Cardiorespiratory/Metabolic Laboratory The University of Memphis Memphis, TN 38152, USA.
Nutr Metab (Lond). 2011 Mar 18;8:17. doi: 10.1186/1743-7075-8-17.
Dietary modification via both caloric and nutrient restriction is associated with multiple health benefits, some of which are related to an improvement in antioxidant status and a decrease in the production of reactive oxygen species. The Daniel Fast is based on the Biblical book of Daniel, is commonly partaken for 21 days, and involves food intake in accordance with a stringent vegan diet. The purpose of the present study was to determine the effect of a 21 day Daniel Fast on biomarkers of antioxidant status and oxidative stress.
43 subjects (13 men; 30 women; 35 ± 1 yrs; range: 20-62 yrs) completed a 21 day Daniel Fast following the guidelines provided by investigators. Subjects reported to the lab in a 12 hour post-absorptive state both pre fast (day 1) and post fast (day 22). At each visit, blood was collected for determination of malondialdehyde (MDA), hydrogen peroxide (H2O2), nitrate/nitrite (NOx), Trolox Equivalent Antioxidant Capacity (TEAC), and Oxygen Radical Absorbance Capacity (ORAC). Subjects recorded dietary intake during the 7 day period immediately prior to the fast and during the final 7 days of the fast.
A decrease was noted in MDA (0.66 ± 0.0.03 vs. 0.56 ± 0.02 μmol L-1; p = 0.004), while H2O2 demonstrated a trend for lowering (4.42 ± 0.32 vs. 3.78 ± 0.21 μmol L-1; p = 0.074). Both NOx (18.79 ± 1.92 vs. 26.97 ± 2.40 μmol L-1; p = 0.003) and TEAC (0.47 ± 0.01 vs. 0.51 ± 0.01 mmol L-1; p = 0.001) increased from pre to post fast, while ORAC was unchanged (5243 ± 103 vs. 5249 ± 183 μmol L-1 TE; p = 0.974). As expected, multiple differences in dietary intake were noted (p < 0.05), including a reduction in total calorie intake (2185 ± 94 vs. 1722 ± 85).
Modification of dietary intake in accordance with the Daniel Fast is associated with an improvement in selected biomarkers of antioxidant status and oxidative stress, including metabolites of nitric oxide (i.e., NOx).
通过热量和营养素限制进行饮食调整与多种健康益处相关，其中一些与抗氧化状态的改善和活性氧产生的减少有关。丹尼尔禁食法基于圣经中的但以理书，通常持续 21 天，并且涉及按照严格的纯素饮食摄入食物。本研究的目的是确定 21 天的丹尼尔禁食对抗氧化状态和氧化应激生物标志物的影响。
43 名受试者（13 名男性；30 名女性；35 ± 1 岁；年龄范围：20-62 岁）按照研究人员提供的指南完成了 21 天的丹尼尔禁食。受试者在禁食前（第 1 天）和禁食后（第 22 天）的 12 小时禁食后到实验室报到。在每次就诊时，采集血液以测定丙二醛（MDA）、过氧化氢（H2O2）、硝酸盐/亚硝酸盐（NOx）、Trolox 等效抗氧化能力（TEAC）和氧自由基吸收能力（ORAC）。受试者在禁食前的 7 天内和禁食的最后 7 天内记录饮食摄入情况。
MDA 降低（0.66 ± 0.0.03 与 0.56 ± 0.02 μmol L-1；p = 0.004），而 H2O2 呈降低趋势（4.42 ± 0.32 与 3.78 ± 0.21 μmol L-1；p = 0.074）。NOx（18.79 ± 1.92 与 26.97 ± 2.40 μmol L-1；p = 0.003）和 TEAC（0.47 ± 0.01 与 0.51 ± 0.01 mmol L-1；p = 0.001）均从禁食前增加到禁食后，而 ORAC 保持不变（5243 ± 103 与 5249 ± 183 μmol L-1 TE；p = 0.974）。正如预期的那样，饮食摄入有多个差异（p < 0.05），包括总热量摄入减少（2185 ± 94 与 1722 ± 85）。
根据丹尼尔禁食法调整饮食摄入与某些抗氧化状态和氧化应激生物标志物的改善有关，包括一氧化氮（即 NOx）的代谢物。
