Salas M A, Evans S W, Levell M J, Whicher J T
Department of Chemical Pathology, Old Medical School, University of Leeds, England.
Clin Exp Immunol. 1990 Mar;79(3):470-3. doi: 10.1111/j.1365-2249.1990.tb08114.x.
We investigated whether interleukin-6 (IL-6) could cause the release of corticosterone by a direct interaction with the adrenal gland. Primary cultures of rat adrenal glands were obtained by dispersion with collagenase and incubated for 24 h with different doses of IL-6. Levels of corticosterone were measured by competitive protein binding assay. A significant (P less than 0.025) dose-dependent increase in corticosterone levels was seen at all doses used. Time course experiments demonstrated that IL-6 stimulated corticosterone release over a period of 24 h but not after 12 or 3 h. The stimulation of adrenal cells with different doses of ACTH1-24 and 40 U/ml of IL-6 showed a synergistic effect when IL-6 was combined with low concentrations of ACTH1-24 (2 and 20 pmol/l). This effect was not evident at higher doses. Our results suggest that IL-6 may act at different levels of the hypothalmic pituitary adrenal axis. Moreover the finding of a synergistic effect with ACTH1-24 indicates that IL-6 could play a role in the long term response to stress.
我们研究了白细胞介素-6（IL-6）是否能通过与肾上腺直接相互作用来促使皮质酮释放。通过用胶原酶分散获得大鼠肾上腺原代培养物，并将其与不同剂量的IL-6孵育24小时。采用竞争性蛋白结合分析法测定皮质酮水平。在所使用的所有剂量下，均观察到皮质酮水平有显著（P小于0.025）的剂量依赖性升高。时间进程实验表明，IL-6在24小时内刺激皮质酮释放，但在12小时或3小时后则无此作用。当IL-6与低浓度的促肾上腺皮质激素1-24（ACTH1-24）（2和20 pmol/l）联合使用时，用不同剂量的ACTH1-24和40 U/ml的IL-6刺激肾上腺细胞显示出协同效应。在较高剂量时这种效应不明显。我们的结果表明，IL-6可能作用于下丘脑-垂体-肾上腺轴的不同水平。此外，与ACTH1-24协同效应的发现表明，IL-6可能在对应激的长期反应中起作用。
