Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan.
BMC Oral Health. 2011 May 13;11:17. doi: 10.1186/1472-6831-11-17.
The aim of this study was to compare the reaction of rat connective tissue to two root-end filling materials: white Mineral Trioxide Aggregate (WMTA) and Diaket.
Each of the materials was placed in dentine tubes and implanted subcutaneously in the dorsal connective tissue of 21 Wistar albino rats. Tissue biopsies were collected 7, 30, and 60 days after the implantation procedure. The specimens were processed and stained with hematoxylin and eosin and examined microscopically. After determining inflammatory cell numbers in sections from each specimen, inflammatory reaction scores were defined as follows: 0; no or few inflammatory cells (no reaction), 1; less than 25 cells (mild reaction), 2; 25 to 125 cells, (moderate reaction), and 3; 125 or more cells (severe reaction). Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney tests.
There were statistically significant differences in the median inflammatory cell numbers throughout the three test periods, with the most severe degree of inflammation observed at the one-week period. Few cases of necrosis were observed with WMTA. Diaket exhibited the most severe degree of inflammation and necrosis. After 30 days, both materials provoked moderate inflammatory reaction. The eight-week period showed the least severe degree of inflammation in all groups.
It was concluded that WMTA exhibits a more favourable tissue response compared with Diaket which induced more severe inflammatory reaction than WMTA and the control.
本研究旨在比较两种根管充填材料（白色矿化三氧化物聚合体和 Diaket）在大鼠结缔组织中的反应。
将每种材料分别置于牙本质管内，然后植入 21 只 Wistar 白化大鼠的背部结缔组织皮下。在植入后 7、30 和 60 天采集组织活检。对标本进行处理和苏木精-伊红染色，并在显微镜下进行检查。在确定每个标本切片中的炎症细胞数量后，将炎症反应评分定义为：0；无或少量炎症细胞（无反应），1；少于 25 个细胞（轻度反应），2；25 至 125 个细胞（中度反应），3；125 个或更多细胞（重度反应）。使用 Kruskal-Wallis 和 Mann-Whitney 检验进行统计分析。
在三个测试期内，炎症细胞数量的中位数存在统计学差异，在一周时观察到最严重的炎症程度。WMTA 中很少有坏死病例。Diaket 表现出最严重的炎症和坏死。30 天后，两种材料均引起中度炎症反应。所有组在八周时显示出最不严重的炎症程度。
与 Diaket 相比，WMTA 表现出更有利的组织反应，后者引起的炎症反应比 WMTA 和对照组更严重。
