相干拉曼扫描光纤内窥镜。
Coherent Raman scanning fiber endoscopy.
机构信息
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
出版信息
Opt Lett. 2011 Jul 1;36(13):2396-8. doi: 10.1364/OL.36.002396.
Abstract
Coherent Raman scattering methods allow for label-free imaging of tissue with chemical contrast and high spatial and temporal resolution. However, their imaging depth in scattering tissue is limited to less than 1 mm, requiring the development of endoscopes to obtain images deep inside the body. Here, we describe a coherent Raman endoscope that provides stimulated Raman scattering images at seven frames per second using a miniaturized fiber scanner, a custom-designed objective lens, and an optimized scheme for collection of scattered light from the tissue. We characterize the system and demonstrate chemical selectivity in mouse tissue images.
摘要
相干拉曼散射方法允许对具有化学对比度和高空间及时间分辨率的组织进行无标记成像。然而，它们在散射组织中的成像深度限制在 1 毫米以内，需要开发内窥镜才能获得体内深处的图像。在这里，我们描述了一种相干拉曼内窥镜，它使用小型光纤扫描仪、定制设计的物镜和优化的方案来收集组织散射光，以每秒 7 帧的速度提供受激拉曼散射图像。我们对该系统进行了特征描述，并在小鼠组织图像中证明了其化学选择性。
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