Segondy M, Vendrell J P, Reynes J, Huguet M F, Albat B, Ducos J, Nicolas J C, Serre A
Laboratoire de Virologie, Hôpital Saint-Eloi, Montpellier, France.
Eur J Clin Microbiol Infect Dis. 1990 Oct;9(10):745-50. doi: 10.1007/BF02184687.
In vitro secretion of antibodies to cytomegalovirus was investigated by analysis of peripheral blood mononuclear cell culture supernatants from subjects infected with cytomegalovirus. Patients with primary or recurrent cytomegalovirus infection showed transient in vitro cytomegalovirus-specific antibody secretion. A high proportion of patients infected with human immunodeficiency virus and sero-positive for cytomegalovirus showed in vitro cytomegalovirus-specific antibody secretion. All peripheral blood mononuclear cell cultures from patients with symptomatic human immunodeficiency virus infection were found to secrete in vitro antibodies to cytomegalovirus, despite the fact that isolation of cytomegalovirus from some of these patients was not achieved. In human immunodeficiency virus-infected patients, in vitro secretion of anti-cytomegalovirus antibodies appeared to be persistent. In vitro cytomegalovirus-specific antibody secretion by peripheral blood lymphocytes probably reflects an in vitro cytomegalovirus-specific B cell activation. This new assay could be considered an interesting method for detecting both acute or chronic cytomegalovirus infection, with potential use in routine laboratory practice.
通过分析巨细胞病毒感染受试者的外周血单个核细胞培养上清液，研究了体外抗巨细胞病毒抗体的分泌情况。原发性或复发性巨细胞病毒感染患者表现出短暂的体外巨细胞病毒特异性抗体分泌。高比例的感染人类免疫缺陷病毒且巨细胞病毒血清学阳性的患者表现出体外巨细胞病毒特异性抗体分泌。尽管从一些有症状的人类免疫缺陷病毒感染患者中未成功分离出巨细胞病毒，但发现所有这些患者的外周血单个核细胞培养物均能在体外分泌抗巨细胞病毒抗体。在人类免疫缺陷病毒感染患者中，体外抗巨细胞病毒抗体的分泌似乎持续存在。外周血淋巴细胞体外巨细胞病毒特异性抗体分泌可能反映了体外巨细胞病毒特异性B细胞活化。这种新检测方法可被视为检测急性或慢性巨细胞病毒感染的一种有趣方法，有在常规实验室实践中应用的潜力。
