Beckwith D G, Halstead D C, Beckwith C H
Diagn Microbiol Infect Dis. 1987 Feb;6(2):145-9. doi: 10.1016/0732-8893(87)90099-x.
Two Cellmatics (Difco) Herpes simplex virus (HSV) detection systems, one with mink lung cells (ML) and the other with primary rabbit kidney cells (PRK) and the Cultureset (Ortho) system with Vero cells were compared for their ability to detect previously positive specimens. One hundred fifty patients specimens positive for HSV in either Vero or PRK cells prior to freezing at -70 degrees C were thawed once, diluted to 1:8, and inoculated in duplicate into each cell system. Positive cultures were detected using each kit's immunoperoxidase method. All three cell lines were stained at 24 hr followed by a final staining of negative cultures at 48 hr for Cultureset and at 72 hr for Cellmatics as per kit instructions. At 24 hr percent detection was: Cellmatics (ML) = 85%, Cellmatics (PRK) = 81%, Cultureset (Vero) = 74%. At final staining percent detection was 94%, 92%, and 84%, respectively. The Cellmatics system has the advantage of fewer steps in the staining procedure and no manipulation of coverslips because staining is performed in the tubes. As employed in these commercial systems, the Cellmatics system with ML cells is more sensitive than the Cultureset with Vero cells (p less than 0.001) and comparable to the Cellmatics system with PRK.
比较了两种Cellmatics（迪福公司）单纯疱疹病毒（HSV）检测系统，一种采用貂肺细胞（ML），另一种采用原代兔肾细胞（PRK），以及带有非洲绿猴肾细胞的Cultureset（奥索公司）系统检测先前阳性标本的能力。150份在-70℃冷冻前在非洲绿猴肾细胞或原代兔肾细胞中HSV呈阳性的患者标本解冻一次，稀释至1:8，并一式两份接种到每个细胞系统中。使用每个试剂盒的免疫过氧化物酶方法检测阳性培养物。按照试剂盒说明，所有三种细胞系在24小时时进行染色，Cultureset系统的阴性培养物在48小时时进行最终染色，Cellmatics系统的阴性培养物在72小时时进行最终染色。在24小时时的检测率为：Cellmatics（ML）=85%，Cellmatics（PRK）=81%，Cultureset（非洲绿猴肾细胞）=74%。在最终染色时的检测率分别为94%、92%和84%。Cellmatics系统的优点是染色过程步骤较少，且无需操作盖玻片，因为染色在试管中进行。在这些商业系统中，采用貂肺细胞的Cellmatics系统比采用非洲绿猴肾细胞的Cultureset系统更敏感（p<0.001），且与采用原代兔肾细胞的Cellmatics系统相当。
