Cunha Valquiria Maria de Paula, Grecco Roseane Lopes da Silva, Paschoini Marina Carvalho, Silva Sueli Riul da, Ruiz Mariângela Torreglosa, Balarin Marly Aparecida Spadotto
Disciplina de Genética,Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brasil.
Rev Bras Ginecol Obstet. 2011 Jul;33(7):158-63. doi: 10.1590/s0100-72032011000700007.
To identify genetic polymorphisms of endothelial growth factor (VEGF), positions +936C/T and -2578C/A, in women with pre-eclampsia.
This was a cross-sectional study conducted on 80 women divided into two groups: pre-eclampsia and control. The sample was characterized using a pre-structured interview and data transcribed from the medical records. DNA extraction, amplification of sequences by the Polymerase Chain Reaction (PCR) with specific primers and polymorphism analysis of Restriction Fragment Length Polymorphism (RFLP) were performed to identify polymorphisms. The statistical analysis was performed in a descriptive manner and using the x² test. The multiple logistic regression model was used to determine the effect of polymorphisms on pre-eclampsia.
A higher frequency of the T allele of the VEGF +936C/T polymorphism was observed in patients with pre-eclampsia, but with no significant difference. The presence of allele A of the VEGF -2578C/A was significantly higher in the control group.
No significant association was observed between VEGF +936C/T polymorphism and pre-eclampsia. For the VEGF -2578C/A polymorphism a significant difference was observed between the control and pre-eclampsia group, with allele A being the most frequent in the control, suggesting the possibility that carriers of allele A have lower susceptibility to the development of pre-eclampsia.
鉴定子痫前期女性中血管内皮生长因子（VEGF）基因多态性，即+936C/T和-2578C/A位点。
这是一项横断面研究，对80名女性进行分组，分为子痫前期组和对照组。通过预先构建的访谈和从病历中转录的数据对样本进行特征描述。进行DNA提取、使用特异性引物通过聚合酶链反应（PCR）扩增序列以及限制性片段长度多态性（RFLP）多态性分析以鉴定多态性。采用描述性统计分析并使用卡方检验。使用多元逻辑回归模型确定多态性对子痫前期的影响。
子痫前期患者中VEGF +936C/T多态性的T等位基因频率较高，但无显著差异。VEGF -2578C/A的A等位基因在对照组中的存在显著更高。
未观察到VEGF +936C/T多态性与子痫前期之间存在显著关联。对于VEGF -2578C/A多态性，在对照组和子痫前期组之间观察到显著差异，A等位基因在对照组中最常见，提示A等位基因携带者患子痫前期的易感性较低。
