College of Marine Life Sciences, Ocean University of China, Qingdao, People's Republic of China.
J Mater Sci Mater Med. 2012 Feb;23(2):457-62. doi: 10.1007/s10856-011-4467-4. Epub 2011 Nov 1.
This experiment was aimed to study whether Carboxymethyl chitosan has acute toxicity and effects on the blood parameters of rats, which were treated with high dosage carboxymethyl chitosan (1350 mg/kg) through a laparotomy. Acute toxicity was first studied and then kinds of blood parameters were detected at different time points after the laparotomy, which contain coagulant parameters (thrombin time, prothrombin time, activated partial thromboplatin time and fibrinogen), anticoagulant parameter (antithrombin III), fibrinolytic parameters (plasminogen and fibrin degradation product) and hemorheology parameters (blood viscosity). Results showed that no acute toxicity was detected and no significant effects were found on the parameters of coagulation, anticoagulation, fibrinolysis or hemorheology of rats after the laparotomy, which indicated that carboxymethyl chitosan has no significant toxicity on the blood system of rats after being absorbed in the abdominal cavity and degraded gradually in the blood. And this study has provided experimental basis for carboxymethyl chitosan to be applied in the field of biomedical materials.
本实验旨在研究羧甲基壳聚糖是否具有急性毒性，以及通过剖腹术给予高剂量羧甲基壳聚糖（1350mg/kg）对大鼠血液参数的影响。首先进行了急性毒性研究，然后在剖腹术后不同时间点检测了各种血液参数，包括凝血参数（凝血酶时间、凝血酶原时间、活化部分凝血活酶时间和纤维蛋白原）、抗凝参数（抗凝血酶 III）、纤维蛋白溶解参数（纤溶酶原和纤维蛋白降解产物）和血液流变学参数（血液黏度）。结果表明，在腹腔吸收并在血液中逐渐降解后，羧甲基壳聚糖对大鼠的血液系统没有明显的毒性，且对大鼠的凝血、抗凝、纤溶或血液流变学参数没有显著影响。这为羧甲基壳聚糖在生物医学材料领域的应用提供了实验依据。
