National Institute for Food and Nutrition Research, Via Ardeatina 546, 00178 Rome, Italy.
Nutr J. 2011 Nov 16;10:125. doi: 10.1186/1475-2891-10-125.
The purpose of this study was to evaluate the overall diet quality effects, mainly on antioxidant nutritional status and some cytokines related to the cellular immune response as well as oxidative stress in a healthy Italian population group.
An observational study was conducted on 131 healthy free-living subjects. Dietary intake was assessed by dietary diary. Standardised procedures were used to make anthropometric measurements. On blood samples (serum, plasma and whole blood) were evaluated: antioxidant status by vitamin A, vitamin E, carotenoids, vitamin C, uric acid, SH groups, SOD and GPx activities; lipid blood profile by total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides; total antioxidant capacity by FRAP and TRAP; the immune status by TNF-α, and IL-10 cytokines; the levels of malondialdehyde in the erythrocytes as marker of lipid peroxidation.
The daily macronutrients intake (g/day) have shown a high lipids consumption and significant differences between the sexes with regard to daily micronutrients intake. On total sample mean Mediterranean Diet Score (MDS) was 4.5 ± 1.6 and no significant differences between the sexes were present. A greater adherence to a Mediterranean dietary pattern increases the circulating plasma levels of carotenoids (lutein plus zeaxanthin, cryptoxanthin, α and β-carotene), vitamin A and vitamin E. The levels of endogenous antioxidants were also improved. We observed higher levels in anti-inflammatory effect cytokines (IL-10) in subjects with MDS ≥ 6, by contrast, subjects with MDS ≤ 3 show higher levels in sense of proinflammatory (TNF α P < 0.05). Lower levels of MDA were associated with MDS > 4. Our data suggest a protective role of vitamin A against chronic inflammatory conditions especially in subjects with the highest adherence to the Mediterranean-type dietary pattern.
Mediterranean dietary pattern is associated with significant amelioration of multiple risk factors, including a better cardiovascular risk profile, reduced oxidative stress and modulation of inflammation.
本研究旨在评估整体饮食质量的影响，主要是针对抗氧化营养状况以及一些与细胞免疫反应和氧化应激相关的细胞因子，在意大利健康人群中进行研究。
对 131 名健康的自由生活受试者进行了一项观察性研究。通过饮食日记评估膳食摄入量。采用标准化程序进行人体测量。评估血液样本（血清、血浆和全血）：通过维生素 A、维生素 E、类胡萝卜素、维生素 C、尿酸、SH 基团、SOD 和 GPx 活性评估抗氧化状态；通过总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、甘油三酯评估血脂谱；通过 FRAP 和 TRAP 评估总抗氧化能力；通过 TNF-α和 IL-10 细胞因子评估免疫状态；通过红细胞中的丙二醛水平作为脂质过氧化的标志物。
每日宏量营养素摄入量（g/天）显示脂质摄入量较高，且男女之间每日微量营养素摄入量存在显著差异。总样本平均地中海饮食评分（MDS）为 4.5±1.6，男女之间无显著差异。更严格地遵循地中海饮食模式会增加循环血浆中叶黄素（叶黄素加玉米黄质、隐黄质、α 和 β-胡萝卜素）、维生素 A 和维生素 E 的水平。内源性抗氧化剂的水平也得到了改善。我们观察到 MDS≥6 的受试者中抗炎作用细胞因子（IL-10）水平较高，而 MDS≤3 的受试者中促炎（TNFα）水平较高（P<0.05）。较低的 MDA 水平与 MDS>4 有关。我们的数据表明维生素 A 对慢性炎症状态具有保护作用，特别是在对地中海饮食模式依从性最高的受试者中。
地中海饮食模式与多种风险因素的显著改善相关，包括心血管风险状况的改善、氧化应激的降低和炎症的调节。
