Biospectrum Life Science Institute, 442-13 Sangdaewon-Dong, Seongnam City, Gyeonggi Do 462-807, Republic of Korea.
Evid Based Complement Alternat Med. 2012;2012:797520. doi: 10.1155/2012/797520. Epub 2011 Nov 1.
Although edible bird's nest (EBN) has been shown to potentiate mitogenic responses, scientific evidence of its efficacy is still limited. In addition, human adipose-derived stem cells (hADSCs) are increasingly accepted as a source for stem cell therapy. Therefore, the aim of this study was to investigate the effects of the EBN extract (EBNE) on the proliferation of hADSCs and its action mechanisms. We found that EBNE strongly promoted the proliferation of hADSCs. In addition, EBNE-induced proliferation was found to be mediated through the production of IL-6 and VEGF, which was induced by activation of AP-1 and NF-κB. Specially, we found that production of IL-6 and VEGF was induced by EBNE. In addition, EBNE-induced production of IL-6 and VEGF was inhibited by PD98059 (a p44/42 MAPK inhibitor), SB203580 (a p38 MAPK inhibitor), and PDTC (a NF-κB inhibitor), but not SP600125 (a JNK inhibitor). Similarly, EBNE-induced proliferation of hADSCs was also attenuated by PD98059, SB203580, and PDTC but not SP600125. Taken together, these findings suggest that the EBNE-induced proliferation of hADSCs primarily occurs through increased expression of IL-6 and VEGF genes, which is mediated by the activation of NF-κB and AP-1 through p44/42 MAPK and p38 MAPK.
尽管燕窝（EBN）已被证明能增强有丝分裂反应，但关于其功效的科学证据仍然有限。此外，人类脂肪来源的干细胞（hADSCs）越来越被认为是干细胞治疗的来源。因此，本研究旨在探讨燕窝提取物（EBNE）对 hADSCs 增殖的影响及其作用机制。我们发现 EBNE 能强烈促进 hADSCs 的增殖。此外，EBNE 诱导的增殖是通过 AP-1 和 NF-κB 的激活诱导产生的 IL-6 和 VEGF 介导的。特别地，我们发现 EBNE 诱导产生了 IL-6 和 VEGF。此外，EBNE 诱导的 IL-6 和 VEGF 的产生被 PD98059（一种 p44/42 MAPK 抑制剂）、SB203580（一种 p38 MAPK 抑制剂）和 PDTC（一种 NF-κB 抑制剂）抑制，但不被 SP600125（一种 JNK 抑制剂）抑制。同样，EBNE 诱导的 hADSCs 增殖也被 PD98059、SB203580 和 PDTC 减弱，但不被 SP600125 减弱。综上所述，这些发现表明，EBNE 诱导的 hADSCs 增殖主要通过增加 IL-6 和 VEGF 基因的表达来发生，这是通过 p44/42 MAPK 和 p38 MAPK 激活 NF-κB 和 AP-1 介导的。
