骆驼垂体阿片促黑皮质素原（阿片肽和促肾上腺皮质激素的共同前体）的纯化至均一性。
Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin.
作者信息
Kimura S, Lewis R V, Gerber L D, Brink L, Rubinstein M, Stein S, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1756-9. doi: 10.1073/pnas.76.4.1756.
Abstract
Pro-opiocortin was purified from camel pituitaries by procedures including high-performance liquid chromatography. The precursor relationship of the pure protein to the opioid peptides and to corticotropin was confirmed. Partial chemical analysis consisting of amino acid analysis and tryptic peptide mapping was carried out with the aid of sensitive fluorescence detection.
摘要
通过包括高效液相色谱在内的方法从骆驼垂体中纯化出阿片皮质素原。证实了该纯蛋白与阿片肽及促肾上腺皮质激素之间的前体关系。借助灵敏的荧光检测进行了包括氨基酸分析和胰蛋白酶肽图谱分析在内的部分化学分析。
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