Department of Life Science, Bhavnagar University, Bhavnagar, 364 002 India.
Indian J Microbiol. 2010 Oct;50(4):369-74. doi: 10.1007/s12088-011-0061-1. Epub 2011 Feb 2.
Acacia catechu, commonly known as catechu, cachou and black cutch is an important medicinal plant and an economically important forest tree. The methanolic extract of this plant was found to have antimicrobial activities against six species of pathogenic and non-pathogenic microorganisms: Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The maximum zone of inhibition (20 mm) was found to be exhibited against S. aureus. For this organism the minimum bactericidal concentration (MBC) of the crude extract was 1,000 μg/ml. The extract was found to be equally effective against gram positive and gram negative bacteria. The antimicrobial activity of the extract was found to be decreased during purification. The chemical constituents of organic plant extracts were separated by thin layer chromatography (TLC) and the plant extracts were purified by column chromatography and were further identified by Gas chromatography-mass selection (GC-MS) analysis. The composition of A. catechu extract had shown major components of terpene i.e. camphor (76.40%) and phytol (27.56%) along with other terpenes in minor amounts which are related with their high antibacterial and antifungal properties.
The online version of this article (doi:10.1007/s12088-011-0061-1) contains supplementary material, which is available to authorized users.
金合欢，通常被称为儿茶，卡楚和黑儿茶是一种重要的药用植物和经济上重要的森林树木。该植物的甲醇提取物被发现对六种致病性和非致病性微生物具有抗菌活性：枯草芽孢杆菌，金黄色葡萄球菌，伤寒沙门氏菌，大肠杆菌，铜绿假单胞菌和白色念珠菌。发现对金黄色葡萄球菌的抑制带（20mm）最大。对于该生物体，粗提取物的最低杀菌浓度（MBC）为 1000μg/ml。提取物对革兰氏阳性和革兰氏阴性细菌同样有效。在纯化过程中发现提取物的抗菌活性降低。通过薄层色谱（TLC）分离有机植物提取物的化学成分，并通过柱色谱对植物提取物进行纯化，并通过气相色谱-质量选择（GC-MS）分析进一步鉴定。儿茶提取物的成分显示出萜烯的主要成分，即樟脑（76.40％）和植醇（27.56％）以及其他萜烯，其含量较少，与它们的高抗菌和抗真菌特性有关。
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