Department of Biomedical Engineering, The Wilmer Eye Institute, The Institute for Nanobiotechnology, and The Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, 400 N Broadway, Smith 5017, Baltimore, MD 21231, USA.
Ann Biomed Eng. 2012 Jul;40(7):1408-18. doi: 10.1007/s10439-012-0550-3. Epub 2012 Mar 27.
Nanoparticles are a promising technology for delivery of new types of therapeutics. A polymer library approach has allowed engineering of polymeric particles that are particularly effective for the delivery of DNA and siRNA to human cells. Certain chemical structural motifs, degradable linkages, hydrophobicity, and biophysical properties are key for successful intracellular delivery. Small differences to biomaterial structure, and especially the type of degradable linkage in the polymers, can be critical for successful delivery of siRNA vs. DNA. Furthermore, subtle changes to biomaterial structure can facilitate cell-type gene delivery specificity between human brain cancer cells and healthy cells as well as between human retinal endothelial cells and epithelial cells. These polymeric nanoparticles are effective for nucleic acid delivery in a broad range of human cell types and have applications to regenerative medicine, ophthalmology, and cancer among many other biomedical research areas.
纳米颗粒是一种很有前途的技术，可用于输送新型治疗药物。聚合物库方法使我们能够设计出对向人体细胞输送 DNA 和 siRNA 特别有效的聚合物颗粒。某些化学结构基序、可降解键、疏水性和生物物理特性是实现成功细胞内输送的关键。生物材料结构的微小差异，尤其是聚合物中可降解键的类型，对于 siRNA 与 DNA 的成功输送可能至关重要。此外，生物材料结构的细微变化可以促进人脑癌细胞与健康细胞之间以及人视网膜内皮细胞与上皮细胞之间的细胞类型基因输送特异性。这些聚合物纳米颗粒在广泛的人类细胞类型中对核酸输送有效，在再生医学、眼科和癌症等许多其他生物医学研究领域都有应用。
