Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.
Curr Eye Res. 2012 Sep;37(9):803-8. doi: 10.3109/02713683.2012.676700. Epub 2012 May 7.
To study whether aqueous humor (AH) and plasma adiponectin (APN) levels were altered in proliferative diabetic retinopathy (PDR).
After excluding subjects with hypertension, hyperlipidaemia, nephropathy, coronary heart disease, heart and renal failure, 20 consecutive type 2 diabetes mellitus (DM) PDR patients and 20 consecutive age-matched non-diabetic and senile cataract (non-DM) controls were enrolled in this study. AH APN and plasma APN concentrations were determined by enzyme-linked immunosorbent assay (ELISA). The expression of AdipoR1/R2 mRNA in peripheral blood mononuclear cells (PBMCs) was measured by real-time polymerase-chain reaction (PCR). The correlations between two parameters, including plasma and AH APN, AdipoR1/R2, plasma insulin and plasma glucose, were analyzed by Pearson's correlation.
In non-DM subjects, the APN concentration in AH was 1.26 ± 0.56 ng/ml, approximately 1/2500 of that in plasma (3.15 ± 1.36 μg/ml). The averaged AH APN concentration in PDR patients was significant higher than that of the non-DM controls (5.29 ± 4.09 ng/ml vs. 1.26 ± 0.56 ng/ml, p < 0.001). Plasma total APN was not correlated with AH APN in the PDR patients, controls or all subjects. No significant difference was observed in plasma total APN levels and AdipoR1/R2 mRNA levels in PBMCs between the PDR patients and the controls (p > 0.05).
Differences in APN concentration between AH and plasma suggest blood-retinal barrier (BRB) plays a key role in maintaining intraocular APN homeostasis. The high APN level in AH may be associated with PDR.
研究增生型糖尿病视网膜病变（PDR）患者房水中（AH）和血浆脂联素（APN）水平是否改变。
排除高血压、高脂血症、肾病、冠心病、心肾衰竭患者后，连续纳入 20 例 2 型糖尿病 PDR 患者（PDR 组）和 20 例年龄匹配的非糖尿病、老年性白内障（非 DM）对照者。采用酶联免疫吸附法（ELISA）测定 AH 和血浆 APN 浓度，实时聚合酶链反应（PCR）测定外周血单个核细胞（PBMC）中 AdipoR1/R2 mRNA 的表达。采用 Pearson 相关分析分析血浆和 AH APN、AdipoR1/R2、血浆胰岛素和血糖之间的相关性。
在非 DM 患者中，AH 中 APN 浓度为 1.26±0.56ng/ml，约为血浆（3.15±1.36μg/ml）的 1/2500。PDR 患者的平均 AH APN 浓度显著高于非 DM 对照组（5.29±4.09ng/ml 比 1.26±0.56ng/ml，p<0.001）。PDR 患者、对照组及所有患者的血浆总 APN 与 AH APN 均无相关性。PDR 患者与对照组的血浆总 APN 水平和 PBMCs 中 AdipoR1/R2mRNA 水平无显著差异（p>0.05）。
AH 与血浆 APN 浓度的差异提示血视网膜屏障（BRB）在维持眼内 APN 内稳态中起关键作用。AH 中高 APN 水平可能与 PDR 有关。
