使用长梯度折射率透镜多光子内镜系统对未染色组织进行体内成像。
In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems.
作者信息
Huland David M, Brown Christopher M, Howard Scott S, Ouzounov Dimitre G, Pavlova Ina, Wang Ke, Rivera David R, Webb Watt W, Xu Chris
出版信息
Biomed Opt Express. 2012 May 1;3(5):1077-85. doi: 10.1364/BOE.3.001077. Epub 2012 Apr 19.
We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable device is capable of imaging a ~200 µm diameter field of view at 4 frames/s. The lateral and axial resolution in water is 0.85 µm and 7.4 µm respectively. In vivo images of unstained tissues in live, anesthetized rats using the portable device are presented. These results show great promise for GRIN endoscopy to be used clinically.
我们对用于多光子成像的长（长达285毫米）梯度折射率（GRIN）透镜内窥镜系统进行了表征。我们制造了一种便携式刚性内窥镜系统，该系统使用直径1毫米、长度8厘米的GRIN透镜系统，适用于对体内潜在深处的未染色组织进行成像。该便携式设备能够以4帧/秒的速度对直径约200微米的视野进行成像。在水中的横向和轴向分辨率分别为0.85微米和7.4微米。展示了使用该便携式设备对活的、麻醉的大鼠体内未染色组织的体内图像。这些结果表明GRIN内窥镜在临床应用方面具有很大的前景。
Zotero 插件