基于对优选供体和受体位点分析的BKV剪接序列。
BKV splice sequences based on analysis of preferred donor and acceptor sites.
作者信息
Seif I, Khoury G, Dhar R
出版信息
Nucleic Acids Res. 1979 Jul 25;6(10):3387-98. doi: 10.1093/nar/6.10.3387.
We have determined the DNA sequences which correspond to the splicing regions in the transcripts of human papovavirus BKV, an evolutionary variant of SV40. To precisely localize the excision points in the BKV sequence, we have conducted a preliminary analysis of numerous viral and eukaryotic splice site sequences. This analysis suggests that the preferred sequence for the donor site belongs to at least one of four groups: Pu↓GTAxG, Pu↓GTAxxT, Pu↓GTxxGT, Pu↓GTxAG (↓ = the cleavage site). These four groups derive from the two basic sequences, Pu↓GTxxG and Pu↓GTA. An optimal donor site might be: AG↓GTAAGT. The preferred sequence for the acceptor site is of the form PyPyxPyAG↓; no dinucleotide AG occurs within 13 nucleotides prior to the terminal AG of the 3' end of the intervening sequences. As we have found in this study of BKV splice sites, the sequences for the preferred donor and acceptor sites provide predictive value in localizing RNA splice points when the DNA sequence is known.
我们已经确定了与人类乳头多瘤空泡病毒BKV（SV40的一种进化变体）转录本中的剪接区域相对应的DNA序列。为了精确确定BKV序列中的切除点，我们对众多病毒和真核生物剪接位点序列进行了初步分析。该分析表明，供体位点的优选序列属于以下四组中的至少一组：Pu↓GTAxG、Pu↓GTAxxT、Pu↓GTxxGT、Pu↓GTxAG（↓ = 切割位点）。这四组源自两个基本序列，Pu↓GTxxG和Pu↓GTA。一个最佳供体位点可能是：AG↓GTAAGT。受体位点的优选序列形式为PyPyxPyAG↓；在间隔序列3'端的末端AG之前的13个核苷酸内不存在二核苷酸AG。正如我们在对BKV剪接位点的这项研究中所发现的，当DNA序列已知时，优选供体和受体位点的序列在定位RNA剪接点方面具有预测价值。
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