University of Mosul, Mosul, Iraq.
Clin Oral Investig. 2013 Apr;17(3):897-904. doi: 10.1007/s00784-012-0751-z. Epub 2012 May 23.
This study was carried out to histologically assess the effect of bone grafting materials extracted from bovine origin on the bone healing process either alone or when mixed with autologous platelet-rich plasma which could be used in many procedures of oral and maxillofacial bone and implant reconstructive surgery.
Sixteen rabbits were used; three intrabony defects in the femur bone of each rabbit were created, one left unfilled for normal healing process and served as control, the second filled with xenogenic graft (Gen-Ox-lyophilized bovine bone organic matrix), and the third filled with (Gen-Ox-lyophilized bovine bone organic matrix) mixed with autologous platelet-rich plasma. Histological examination of the sections was performed after staining with H&E and Van Geison stains. The histomorphometric analysis including counting of bone cells (osteoblasts, osteocytes, and osteoclasts) with performance of osteon diameter and lamellar thickness at the end of the fourth week postoperatively was obtained.
It has been shown that with the use of autologous platelet-rich plasma in combination with the xenogenic bone graft prepared from bovine origin, new bone formation and neovascularization were enhanced significantly when compared with xenogenic graft alone.
The addition of PRP to xenogenic bone substitute in small bone defects of the rabbit femur showed a histomorphometric increase in bone formation (at the fourth week of healing).
Platelet concentrate might be used to accelerate the osseointegration of enosseous dental implants.
本研究旨在通过组织学评估从牛源性提取的骨移植材料对骨愈合过程的影响，无论是单独使用还是与自体富血小板血浆混合使用，均可用于口腔颌面骨和种植体重建手术的许多程序。
使用了 16 只兔子；在每只兔子的股骨中创建了三个骨内缺损，一个未填充以用于正常愈合过程作为对照，第二个填充了异种移植物（Gen-Ox-冻干牛骨有机基质），第三个填充了（Gen-Ox-冻干牛骨有机基质）与自体富血小板血浆混合。用 H&E 和 Van Geison 染色后对切片进行组织学检查。在术后第四周，通过计数成骨细胞（成骨细胞、骨细胞和破骨细胞）、进行骨单位直径和板层厚度的组织形态计量分析，获得了组织形态计量学分析。
结果表明，与单独使用异种移植物相比，自体富血小板血浆与牛源性异种骨移植物联合使用可显著增强新骨形成和新生血管形成。
在兔股骨小骨缺损中，将 PRP 添加到异种骨替代物中，在第 4 周的愈合时显示出骨形成的组织形态计量学增加。
血小板浓缩物可用于加速骨内牙种植体的骨整合。
