Center for Research at Bio/nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, University of Florida , Gainesville, Florida 32611-7200, United States.
ACS Appl Mater Interfaces. 2012 Jun 27;4(6):3007-11. doi: 10.1021/am300374q. Epub 2012 Jun 18.
In this work, gold nanoparticles perform Boolean logic operations in response to two proangiogenic targets important in cancer diagnosis and treatment: PDGF and VEGF. In the absence of protein target, gold nanoparticles are initially dispersed as a red solution; the addition of target proteins causes nanoparticle aggregation, turning the solution blue, as well as the release of dye-labeled aptamer probes, which causes an increase in fluorescence. These outputs constitute an AND or OR gate for simultaneous protein detection. We believe this logic-gate-based detection system will become the basis for novel rapid, cheap, and reliable sensors for diagnostic applications.
在这项工作中，金纳米粒子对两种在癌症诊断和治疗中非常重要的促血管生成靶点 PDGF 和 VEGF 做出反应，从而执行布尔逻辑运算。在没有蛋白质靶标的情况下，金纳米粒子最初作为红色溶液分散；靶蛋白的加入会导致纳米粒子聚集，使溶液变为蓝色，并释放出带有荧光染料标记的适体探针，从而导致荧光强度增加。这些输出构成了用于同时检测蛋白质的与门或或门。我们相信，这种基于逻辑门的检测系统将成为用于诊断应用的新型快速、廉价且可靠传感器的基础。
