Regional Blood Donation Centre and University of Murcia, Spain.
Blood Transfus. 2012 Oct;10(4):480-9. doi: 10.2450/2012.0112-11. Epub 2012 Apr 13.
A novel and practical storage container designed for manual buffy-coat pooling and leucodepletion was evaluated to assess its filtration performance and to analyse the quality of stored leucoreduced buffy-coat-derived platelet pools.
To analyse the grifols leucored transfer PL system, blood was collected from random donors into standard triple bag systems, and fractionated using standard procedures to obtain buffy-coats. Ten leucodepleted platelet pools were prepared each from five units of buffy-coats in additive solution. Concentrates were stored for 10 days at 22 °C on an end-over-end agitator. On days 0, 5, 7, and 10 of storage, samples were tested using standard in vitro platelet parameters.
The use of this novel system for volume reduction and leucodepletion of buffy-coats resuspended in additive solution led to platelet pools that met the European requirements. pH was maintained well, declining from an initial value of 7.11±0.04 to 6.88±0.08 after 10 days. Parameters of cell lysis, response to a hypotonic stimulus and aggregation induced by agonists (arachidonic acid, ristocetin, collagen or thrombin receptor activating peptide) were also well-preserved. During storage, the quality profile of the platelet pools remained very similar to that previously reported in platelet concentrates in terms of metabolism, platelet activation (CD62, CD63, sCD62), expression of glycoproteins Ib and IIb/IIIa, capacity of glycoprotein IIb/IIIa to become activated upon ADP stimulation, and release of biological response modifiers (sCD40L and RANTES).
This new system allows the preparation of leucodepleted buffy-coat platelet pools in additive solution with good preservation of platelet function. The logistics of the procedure are relatively simple and it results in good-quality components, which may reduce costs and ease the process of buffy-coat pooling and leucocyte reduction in transfusion services.
为了评估一种新型实用的手动制备混合浓缩血小板并去除白细胞的储存容器的过滤性能，分析储存后去白细胞混合浓缩血小板的质量，我们对其进行了研究。
采用格里菲斯白细胞去除转移 PL 系统，采集随机献血者的血液至标准三联袋系统中，按照标准程序分离得到富血小板血浆。采用添加剂溶液悬浮白细胞去除后的富血小板血浆，每份由 5 单位富血小板血浆制备，制备 10 份去白细胞浓缩血小板。浓缩血小板在 22°C 的水平旋转式搅拌器上储存 10 天。在储存的第 0、5、7 和 10 天，使用标准的体外血小板参数对样本进行测试。
使用该新型系统对悬浮于添加剂溶液中的富血小板血浆进行体积减少和白细胞去除处理后，制备的血小板符合欧洲标准。pH 值保持良好，初始值为 7.11±0.04，10 天后降至 6.88±0.08。细胞溶解、对低渗刺激的反应以及用激动剂（花生四烯酸、瑞斯托霉素、胶原或血栓素受体激活肽）诱导的聚集参数也得到了很好的保留。在储存过程中，与之前报道的血小板浓缩物相比，血小板池的质量特征在代谢、血小板激活（CD62、CD63、sCD62）、糖蛋白 Ib 和 IIb/IIIa 的表达、ADP 刺激后糖蛋白 IIb/IIIa 的激活能力以及生物反应调节剂（sCD40L 和 RANTES）的释放等方面保持非常相似。
该新型系统允许在添加剂溶液中制备去白细胞富血小板血浆，并能很好地保留血小板功能。该程序的后勤工作相对简单，并且可以获得高质量的成分，这可能会降低成本并简化输血服务中富血小板血浆的制备和白细胞去除的过程。
