木豆叶和根乙醇提取物与传统抗真菌剂之间的抗真菌活性评估及调节作用
Evaluation of the antifungal activity and modulation between Cajanus cajan (L.) Millsp. leaves and roots ethanolic extracts and conventional antifungals.
作者信息
Brito Samara A, Rodrigues Fabíola F G, Campos Adriana R, da Costa José G M
机构信息
Molecular Bioprospection Post-graduation Program, Laboratory of Natural Products Research, Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil.
出版信息
Pharmacogn Mag. 2012 Apr;8(30):103-6. doi: 10.4103/0973-1296.96550.
BACKGROUND
The use and investigation of natural products with antimicrobial activity from vegeral source have been reported by several researchers. Cajanus cajan (Fabaceae) is a multiple use specie mainly as human food. In popular medicine, diverse parts of the plant are used as sedative and to treat cough, hepatitis, and diabetes.
MATERIALS AND METHODS
This study shows the characterization of secondary metabolites present in ehtanolic extracts from leaves and roots of Cajanus cajan by phytochemical prospection. The evaluation of the antifungal activity was performed by the microdilution method, and from the subinhibitory concentrations (MIC 1/8) the modulatory activity of antifungical (fluconazole and ketoconazole) was analyzed by the direct contact assay against C. albicans ATCC40006, Candida krusei ATCC 6538 and Candida tropicalis ATCC 40042.
RESULTS
The results showed the presence of tannins, flavonoids, and alkaloids in both extracts as the clinically relevant antifungal activity. The modulatory potential is presented by the antifungal tested against yeasts.
CONCLUSION
The extracts studied here have demonstrated to be a new therapeutic source to treat these microorganism-associated diseases.
背景
几位研究人员报告了对具有抗菌活性的植物源天然产物的使用和研究。木豆（豆科）是一种具有多种用途的物种，主要作为人类食物。在传统医学中，该植物的不同部位被用作镇静剂，并用于治疗咳嗽、肝炎和糖尿病。
材料与方法
本研究通过植物化学勘探对木豆叶和根的乙醇提取物中存在的次生代谢产物进行了表征。通过微量稀释法评估抗真菌活性，并从亚抑制浓度（MIC 1/8）通过直接接触试验分析抗真菌剂（氟康唑和酮康唑）对白色念珠菌ATCC40006、克鲁斯念珠菌ATCC 6538和热带念珠菌ATCC 40042的调节活性。
结果
结果表明，两种提取物中均存在单宁、黄酮类化合物和生物碱，具有临床相关的抗真菌活性。所测试的抗真菌剂对酵母具有调节潜力。
结论
此处研究的提取物已证明是治疗这些微生物相关疾病的新治疗来源。
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