Hu Runlei, Ma Shenglin, Li Hu, Ke Xianfu, Wang Guoqing, Wei Dongshan, Wang Wei
Department of Thoracic Surgery, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, P.R. China.
Oncol Lett. 2011 Nov;2(6):1161-1164. doi: 10.3892/ol.2011.379. Epub 2011 Aug 9.
The purpose of the present study was to investigate the therapeutic effect of magnetic fluid hyperthermia (MFH) induced by an alternating magnetic field (AMF) on human carcinoma A549 xenograft in nude mice. An animal model of human lung cancer was established by subcutaneous injection of human lung cancer A549 cells in BALB/c nude mice. The xenograft mice were randomly divided into four groups and each group was treated with an injection of a different concentration of magnetic fluid: control, low-dose (67.5 mg/ml), medium-dose (90.0 mg/ml) and high-dose group (112.5 mg/ml), respectively. Following the injection (24 h), the tumor was heated in an AMF for 30 min. Tumor volumes were then measured every week. The therapeutic effect was assessed by measuring the tumor volume and weight. Pathological examination was performed with a light and electronic microscope following treatment. The temperature at the surface of the tumor in the low-, medium- and high-dose groups increased to 41.3, 44.5 and 46.8°C, respectively. The tumor grew significantly slower in the medium- and high-dose groups (both p<0.05) compared to the control group. Cytoclasis and apoptosis were detected under light and electron microscopy. In conclusion, MFH induced by AMF inhibited tumor growth and promoted apoptosis of human carcinoma A549 cells in a xenograft mice model.
本研究的目的是探讨交变磁场（AMF）诱导的磁流体热疗（MFH）对裸鼠人癌A549异种移植瘤的治疗效果。通过在BALB/c裸鼠皮下注射人肺癌A549细胞建立人肺癌动物模型。将异种移植瘤小鼠随机分为四组，每组分别注射不同浓度的磁流体：对照组、低剂量组（67.5 mg/ml）、中剂量组（90.0 mg/ml）和高剂量组（112.5 mg/ml）。注射后（24小时），在交变磁场中将肿瘤加热30分钟。然后每周测量肿瘤体积。通过测量肿瘤体积和重量评估治疗效果。治疗后用光学显微镜和电子显微镜进行病理检查。低、中、高剂量组肿瘤表面温度分别升至41.3、44.5和46.8°C。与对照组相比，中、高剂量组肿瘤生长明显较慢（均p<0.05）。在光学显微镜和电子显微镜下检测到细胞破碎和凋亡。总之，交变磁场诱导的磁流体热疗在异种移植瘤小鼠模型中抑制了人癌A549细胞的肿瘤生长并促进了其凋亡。
