Lachoo Memorial College of Science and Technology, Pharmacy Wing, Jodhpur 342008, Rajasthan, India.
J Ethnopharmacol. 2012 Dec 18;144(3):786-90. doi: 10.1016/j.jep.2012.10.006. Epub 2012 Oct 10.
[corrected] Euphorbia caducifolia is a Euphorbiaceae species native to Thar Desert of India, where latex of E. caducifolia (ECL) is used by the local inhabitants for treatment of bleeding wound, cutaneous eruption and other skin diseases. Looking to the medicinal importance of the plant, it was considered worthwhile to scientifically evaluate the ECL for the wound healing activity.
In vitro methods, clotting of platelet free plasma and angiogenesis in chick chorioallantoic membrane (CCM) were used for studying effect of ECL on clotting and angiogenesis. Excision and incision wounds model were used to study effect of ECL on wound contraction, tensile strength and hydroxyproline and DNA content.
ECL treatment stimulates the blood vessel formation in CCM, at the all dose levels. 56.77, 74.48 and 78.09% increase in area of angiogenesis was observed in ECL 2.5, 5.0 and 10mg/ml treated groups respectively. The excised skin of ECL 5.0 and 10mg/ml treated animals found to have higher content of hydroxyproline and DNA, when compared with control group. An acceleration of the wound closure was observed with an enhanced number of fibroblasts and collagen content in ECL treated mice.
Result of in vitro and in vivo experiments indicate that ECL exerts significant wound healing activity, demonstrating its relevant therapeutic potential.
Euphorbia caducifolia 是一种原产于印度塔尔沙漠的大戟科物种，当地居民使用其乳胶（ECL）来治疗出血伤口、皮肤疹和其他皮肤病。鉴于该植物的药用重要性，有必要对 ECL 的伤口愈合活性进行科学评估。
采用体外方法，即无血小板血浆的凝结和鸡胚绒毛尿囊膜（CCM）中的血管生成，研究 ECL 对凝结和血管生成的影响。采用切除和切口伤口模型，研究 ECL 对伤口收缩、拉伸强度以及羟脯氨酸和 DNA 含量的影响。
ECL 处理可刺激 CCM 中的血管形成，在所有剂量水平下均如此。在 ECL 2.5、5.0 和 10mg/ml 处理组中，血管生成面积分别增加了 56.77%、74.48%和 78.09%。与对照组相比，ECL 5.0 和 10mg/ml 处理动物的切除皮肤中羟脯氨酸和 DNA 的含量更高。在 ECL 处理的小鼠中，观察到伤口闭合加速，成纤维细胞数量增加，胶原含量增加。
体外和体内实验的结果表明，ECL 具有显著的伤口愈合活性，显示出其相关的治疗潜力。
