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免疫层析抗原检测法检测粪便标本中非空肠弯曲菌和大肠弯曲菌。

Detection of non-jejuni and -coli Campylobacter species from stool specimens with an immunochromatographic antigen detection assay.

机构信息

Associated Regional and University Pathologists Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.

出版信息

J Clin Microbiol. 2013 Jun;51(6):1935-7. doi: 10.1128/JCM.03208-12. Epub 2013 Apr 3.

Abstract

The STAT! Campy immunochromatographic assay for Campylobacter antigen was compared to culture for 500 clinical stool specimens. Antigen was detected in six culture-negative, PCR-positive specimens. C. upsaliensis, a pathogenic species that is traditionally difficult to recover in routine stool cultures, was detected in two of these culture-negative specimens. This study provides evidence that antigen testing may cross-react with at least one additional non-jejuni and -coli Campylobacter species that may be missed by routine culture for campylobacteriosis.

摘要

STAT! Campy 免疫层析检测试剂盒用于检测粪便标本中的弯曲菌抗原,与培养法相比,对 500 份临床粪便标本进行了检测。在 6 份培养阴性、PCR 阳性的标本中检测到了抗原。在这 6 份培养阴性的标本中,有 2 份检测到了传统上难以从常规粪便培养中恢复的致病性物种 C. upsaliensis。本研究提供了证据表明,抗原检测可能与至少一种其他非空肠弯曲菌和结肠弯曲菌种发生交叉反应,这些种可能会被常规的弯曲菌病培养所遗漏。

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