Department of Obstetrics and Gynecology, Hospital Sant Joan de Déu, Faculty of Medicine-University of Barcelona, Barcelona, Spain.
J Ovarian Res. 2013 May 7;6(1):33. doi: 10.1186/1757-2215-6-33.
Currently, cryopreservation of oocytes, embryos and ovarian tissue is considered the basis of fertility preservation programs for women with cancer and other diseases who are rendered sterile by gonadotoxic drugs or radiation.Numerous studies have confirmed that autograft of frozen-thawed ovarian tissue can restore ovarian function and fertility. A total of twenty-two live births have been reported but we still have to consider this technique as experimental. The main problem is that the implant undergoes ischemia until neoangiogenesis is restored, resulting in significant follicular loss.At the moment, there are numerous publications in different medical fields that publish successful experiences with plasma rich in platelets (PRP) in different clinical situations promoting angiogenesis. Thus, we considered the possibility of using it in the field of ovarian autologous transplantation in order to improve the vascularization of the implant and its quality. For this, both thawed ovarian tissue as practiced pockets on the rear side of the broad ligament which have been placed, have been impregnated with PRP. We can say that the implant treated in this way has had a rapid and successful response.We report a special interesting case because this is the first time that this technique is performed successfully in a woman without ovaries combined with growth factors to promote neoangiogenesis. Obviously, the results of the hormonal response come exclusively from the implanted tissue in these special conditions.
目前，卵母细胞、胚胎和卵巢组织的冷冻保存被认为是癌症和其他疾病女性生育力保存计划的基础，这些女性因性腺毒性药物或辐射而不育。大量研究证实，冷冻-解冻卵巢组织的自体移植可以恢复卵巢功能和生育能力。已经报道了总共 22 例活产，但我们仍将这项技术视为实验性的。主要问题是，植入物会经历缺血，直到新血管生成恢复，导致大量卵泡损失。目前，许多不同医学领域的出版物都公布了富含血小板的血浆（PRP）在不同临床情况下促进血管生成的成功经验。因此，我们考虑将其用于卵巢自体移植领域，以改善植入物的血管化及其质量。为此，我们用 PRP 浸泡解冻的卵巢组织和已放置在阔韧带后侧的手术口袋。我们可以说，这种方法处理的植入物反应迅速且成功。我们报告了一个特别有趣的病例，因为这是第一次在没有卵巢的女性中成功地将这种技术与生长因子结合使用，以促进新血管生成。显然，在这些特殊条件下，激素反应的结果完全来自植入的组织。
