Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
Biomed Res Int. 2013;2013:926942. doi: 10.1155/2013/926942. Epub 2013 Apr 29.
Consumption of isoflavones may prevent adiposity, hepatic steatosis, and dyslipidaemia. However, studies in the area are few and primarily with genistein. This study investigated the effects of formononetin and its synthetic analogue, 2-heptyl-formononetin (C7F), on lipid and cholesterol metabolism in C57BL/6J mice. The mice were fed a cholesterol-enriched diet for five weeks to induce hypercholesterolemia and were then fed either the cholesterol-enriched diet or the cholesterol-enriched diet-supplemented formononetin or C7F for three weeks. Body weight and composition, glucose homeostasis, and plasma lipids were compared. In another experiment, mice were fed the above diets for five weeks, and hepatic triglyceride accumulation and gene expression and histology of adipose tissue and liver were examined. Supplementation with C7F increased plasma HDL-cholesterol thereby increasing the plasma level of total cholesterol. Supplementation with formononetin did not affect plasma cholesterol but increased plasma triglycerides levels. Supplementation with formononetin and C7F induced hepatic steatosis. However, formononetin decreased markers of inflammation and liver injury. The development of hepatic steatosis was associated with deregulated expression of hepatic genes involved in lipid and lipoprotein metabolism. In conclusion, supplementation with formononetin and C7F to a cholesterol-enriched diet adversely affected lipid and lipoprotein metabolism in C57BL/6J mice.
摄入异黄酮可能预防肥胖、肝脂肪变性和血脂异常。然而，该领域的研究较少，且主要集中在染料木黄酮上。本研究调查了芒柄花素及其合成类似物 2-庚基芒柄花素（C7F）对 C57BL/6J 小鼠脂质和胆固醇代谢的影响。小鼠用富含胆固醇的饮食喂养五周以诱导高胆固醇血症，然后用富含胆固醇的饮食或富含胆固醇的饮食补充芒柄花素或 C7F 喂养三周。比较体重和成分、葡萄糖稳态和血浆脂质。在另一个实验中，小鼠用上述饮食喂养五周，检查肝组织甘油三酯积累和基因表达以及脂肪组织和肝脏的组织学。C7F 的补充增加了血浆高密度脂蛋白胆固醇，从而增加了总胆固醇的血浆水平。芒柄花素的补充并不影响血浆胆固醇，但增加了血浆甘油三酯水平。芒柄花素和 C7F 的补充诱导了肝脂肪变性。然而，芒柄花素降低了炎症和肝损伤的标志物。肝脂肪变性的发展与参与脂质和脂蛋白代谢的肝基因表达失调有关。总之，在富含胆固醇的饮食中补充芒柄花素和 C7F 会对 C57BL/6J 小鼠的脂质和脂蛋白代谢产生不利影响。
